摘要
目的：测定牛蒡植株不同部位总黄酮的含量。方法：采用L9（3。）正交设计试验，对提取时间、料液比、乙醇浓度、提取次数4因素进行考察，以确定最佳提取工艺；以芦丁为对照，用亚硝酸钠-硝酸铝-氢氧化钠比色法测定总黄酮含量。结果：最佳提取工艺为：乙醇浓度55％，料液比1：70，提取时间1．5h，提取次数2次；芦丁在4．00～100．00μg／mL范围内线性关系良好，r=0．9998，总黄酮平均回收率为97．7％，RSD为2．5％（n=6）。结论：该提取工艺稳定可行，含量测定方法准确。
Objective： To determine the content of the total flavones from the Arctium lappa L.. Methods： The best extraction technology was determined by orthogonal experiment design, including extraction time, solid-liquid ratio, ethanol concentration and the extraction times. The content of total flavones was determined by absorption spectrometry with rutosids as reference substance. Results： Optimum extraction technology was as follows： 55% ethanol,ratio of material to liquid 1：70,time 1.5 h,two times. The calibration curve had good linear relationship in the range of 4.00-100.00 μg/mL. The average recovery was 97.7%,RSD=2.5%（n=6）. Conclusion： The extraction process is stable and feasible. The quantitative method is accurate.
出处
《大理学院学报（综合版）》
CAS
2013年第12期12-15,共4页
Journal of Dali University
基金
大理学院大学生科研基金资助项目(YSKY201206)
关键词
牛蒡
总黄酮
提取工艺
含量测定
A rctiura lappa L.
total flavones
extraction technology
contents determination
