摘要
目的:探讨酸枣仁原药及其多糖、生物碱的抗抑郁作用。方法:建立小鼠抑郁症模型,用酸枣仁水煎液、酸枣仁多糖、酸枣仁生物碱和盐酸氟西汀对抑郁症模型进行灌胃治疗,通过强迫游泳实验和悬尾实验评价各组药物疗效。实验结束,取脑,作组织匀浆,测定脑组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果:与空白组比较,模型组小鼠呈明显抑郁状态,强迫游泳实验和悬尾实验中模型组不动时间较长(P<0.01),SOD活性降低(P<0.01),MDA含量升高(P<0.01);与模型组比较,阳性对照组、酸枣仁水煎液组、酸枣仁生物碱组小鼠游泳和悬尾不动时间均减少(P<0.05,P<0.01),SOD活性升高(P<0.05,P<0.01),MDA含量降低(P<0.05,P<0.01)。酸枣仁多糖组变化不明显。结论:酸枣仁有抗抑郁、镇静的作用,其中生物碱抗抑郁作用较强,多糖类抗抑郁作用不明显,且药效均不及酸枣仁煎剂。
Objective： To observe anti depression effect of active ingredients in Semen Ziziphi spinosae. Methods ： The de- pression mice models were established by chronic unpredicted mild stress （CUMS） for successive 24 days. The mice in different groups were administered Semen Ziziphi spinosae decoction,Semen Ziziphi spinosae alkaloid,Semen Ziziphi spinosae polysaccharides and fluoxertine hydrochloride respectively. Then activity of superoxide dismutase （SOD） and con- tent of malonaldehyde （MDA） in brain tissue of mice were examined. Results：Compared with the blank control group, im- mobility time was increased in forced swimming test and tail suspension test （P〈0.01）, the activity of SOD was decreased （P〈0.01）,the content of MDA was increased in model group （P〈0.01）. Compared with model group,immobility time was decreased（P〈0.05 ,P〈0.01 ）, activity of SOD was inereased（P〈0.05,P〈0.01 ）,content of MDA was decreased in treatment groups（P〈0.05, P〈0.01 ）. Conclusion：Semen Ziziphi spinosae has anti depression effect. Its active ingredients includes al- kaloid and polysaceharides. However the Semen Ziziphi spinosae alkaloid or polysaccharides separately is less effective than Semen Ziziphi spinosae decoction.
出处
《山西中医学院学报》
2013年第5期16-18,共3页
Journal of Shanxi College of Traditional Chinese Medicine
基金
太原市中药与生物新制剂研发平台项目(2012-3-PT-F-125)
关键词
酸枣仁
生物碱
抗抑郁
应激抑郁模型
Semen Ziziphi spinosae
alkaloids
antidepression effect
stress depression model
