摘要
用红外光谱内标法（K3[Fe（CN）6）]·6H2O为内标物）测定了红霉素肠溶片中红霉素的含量.实验表明：混合物红外光谱中红霉素的测量峰（1714.6cm-1）和K3[Fe（CN）6）]·6H2O的内标峰（2119.0cm-1）的峰高比（y=h i/h s）与两者质量比（x=m i/m s）有良好的线性关系,其线性回归方程为y=0.2642x-0.0579,相关系数R2=0.9973.据此测得红霉素肠溶片中红霉素的含量为标示量的101%,相对标准偏差为0.51%,加标回收率分别为96.8%-101.9%.该方法无需分离提取,简便,快速,成本低,测定结果准确,特别适合批量检测和药品质量的快筛,有应用价值.
Potassium ferricyanide was added into erythromycin samples as internal reference and the mixtures were measured by Fourier transform infrared spectrophotometry（ FT- IR）. The peak of erythromycin at 1714. 6 cm- 1 was chosen as quantitative peak and the one of potassium ferricyanide at 2119. 0 cm- 1 was chosen as inside mark quantitative peak. The ratio of two peak＇s absorbance（ y = h i / h s） had good linear relationship with their mass ratio（ x = m i / m s）. The linear regression equation was y = 0. 2642x- 0. 0579 with the correlation coefficient R2 = 0. 9973. The contents of erythromycin in the samples of erythromycin enteric- coated tablets was 101%,and the relatively standard deviation was 0. 51%,and the recoveries of the method was 96. 8% ~ 101. 9%. This method does not require separation and extraction,especially for batch testing and drug quality rapid screening.
出处
《嘉应学院学报》
2013年第11期36-39,共4页
Journal of Jiaying University
基金
国家自然科学基金资助项目(21101077)
关键词
红外光谱内标法
红霉素肠溶片
红霉素
internal standard method of FTIR
erythromycin enteric-coated tablets
erythromycin
