摘要
目的建立测定三金颗粒中羟基积雪草苷含量的高效液相色谱（HPLC）法。方法采用C,8柱（250 mm x 4. 6 mm,5 μm）以乙腈- 2 mmol/L p -环糊精溶液（24 ： 76）为流动相，柱溫35 t，流速1.0 mL/min,检测波长205 nm。结果羟基积雪草苷进样量在0. 218 8 - 21. 884 3 （xg范围内与峰面积积分值线性关系良好（r =0. 999 9），平均回收率为103. 08% , RSD为1. 22% U = 9）。结论该方法重复性、准确性良好，可用于三金颗粒中羟基积雪草苷的含量测定。
Objective To establish a HPLC method for the content determination of madecassoside in Sanjin Granules. Methods The Cig column（250 mm x4. 6 mm,5 fun） was used with acetonitrile - P - cyclodextrin（24 ： 76,2 mmol/L） as the mobile phase at 35 °C. The flow rate was 1.0 mL/ min and the detection wavelength was set at 205 nm Results The calibration curve of madecassoside was good linear in the range of 0.218 84-21. 884 3 p,g （ r =0. 999 9） ,and the average recovery rate was 103. 08% with RSD 1. 22% （ n -9）. Conclusion This method is good in the accuracy and the repeatability and can be applied to the content determination of madecassoside in Sanjin Granule.
出处
《中国药业》
CAS
2014年第2期50-51,共2页
China Pharmaceuticals
