摘要
对一批美国进境的非种用黄大豆和夹带的大豆茎秆进行了病原菌分离,在大豆茎秆上分离到1株疑似葡萄茎枯病菌的菌株Pg-84302。通过对该菌株进行病原菌形态学观察、致病性测定并结合分子生物学方法检测,结果表明菌株Pg-84302在PDA培养基和大豆茎秆上均产生大量分生孢子器、分生孢子和砖格状厚垣孢子,分生孢子器通常为球形;经真菌通用引物扩增和测序,其与GenBank中Phoma glomerata序列(登录号:EU098115.1、AF126819.1、EU273521.1、AY183371.1)的同源性为100%;该菌不仅能侵染大豆,还侵染小麦和苹果。根据上述实验结果,将分离获得的菌株Pg-84302鉴定为葡萄茎枯病菌(Phoma glomerata(Corda)Wollenweber et Hochapfel)。
The isolate Pg-84302 was isolated on imported soybean impurities from American in Shanghai port by traditional PDA method. The isolate Pg-84302 grew well on PDA and soybean stalks, producing massive pycnidia, conidia and chlamydospores. The pycnidia were usually spheroid. The isolate DNA was amplificated and sequenced by universal primers. Compared with the identity of rDNA sequence of Phoma glomerata （Accession： EU098115.1, AF126819.1, EU273521.1, AY183371.1） from NCBI GenBank databases by blast method, the result showed that its homology was up to 100%. Pathogenicity tests showed that soybean, wheat and apple were infected by Pg-84302. Based on morphological characteristics, molecular and pathogenicity test results, the isolate Pg-84302 was identified as Phoma glomerata （Corda） Wollenweber ＆ Hochapfel.
出处
《植物检疫》
北大核心
2014年第1期48-52,共5页
Plant Quarantine
基金
上海市科委技术标准专项(11dz0502700)
上海检验检疫局科技项目(HK010-2012)
关键词
葡萄茎枯病菌
形态特征
序列分析
致病性测定
鉴定
Phoma glomerata
morphological characteristics
molecular detection
pathogenicity
identification
