摘要
利用密闭环境控制室熏气处理,研究了不同浓度(自然状态和0.5、1.5、3.0mg·L-1)SO2对盆栽巨桉、天竺桂、西蒙得木和茶树幼树叶片渗透调节物质含量、抗氧化系统保护酶活性和丙二醛含量的影响,并就各树种对SO2的抗性进行综合评价。结果显示:(1)SO2对4个树种叶片伤害程度表现为天竺桂<西蒙得木<巨桉<茶树。(2)SO2胁迫显著增加了巨桉和西蒙得木叶片可溶性蛋白(SP)、可溶性糖(SS)和游离脯氨酸(Pro)3种渗透调节物质含量;SO2胁迫对天竺桂叶片SP和SS含量无显著影响,且对Pro含量的促进作用也是在处理30d后才体现出来;SO2胁迫对茶树叶片SP含量无显著影响,使SS含量显著降低,而使Pro含量显著增加。(3)SO2胁迫总体使巨桉和天竺桂抗氧化系统保护酶———超氧化物酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性和丙二醛(MDA)含量增加,但西蒙得木各指标表现不同,而使茶树抗氧化系统保护酶活性和丙二醛含量则全面下降。(4)巨桉、天竺桂、西蒙得木、茶树的最大净吸收S量依次为1.17、1.32、2.04、0.95g·kg-1。(5)通过隶属函数法综合7个生理指标得到的SO2抗性综合排序为天竺桂>西蒙得木>巨桉>茶树,与叶片伤害程度表现一致。研究表明,植物抗氧化保护酶系统在4个树种抵抗SO2胁迫调节机制中具有重要作用,其中天竺桂超高的基础POD活性可能是其抵抗SO2伤害的关键机制之一。
Resistance physiological indexes such as soluble proteins（SP）, soluble sugars（SS）,free proline （Pro） ,superoxide dismutase （SOD）,peroxidase （POD）,catalase （CAT） and malondialdehyde （MDA） of four species of samplings： Eucalyptus grandis （Eg）, Cinnamomum pedunculatum （Cp）, Simmondisa chinensis （Sc） and Camellia sinensis （Cs） were measured after fumigation of different levels of SO2 in sealed environmental control chambers. Four levels of SOz treatments：control （natural concentration）,M1 （0.5 mg. L^-1）,M2 （1.5 mg . L^-1）,and M3 （3.0 mg . L^-1） were set in the present study. The results showed that. （1）The order of the extent of injury of SO2 on leaves is：Cp〈Sc〈Eg〈Cs. （2）SO2 treatment significantly increased the concentrations of SP,SS,and Pro in leaves of Eg and Sc. There was no significant effect of SO2 stress on SP and SS of Cp. The stimulated effect of SO2 stress on Pro of Cp appeared only on 30 d of treatment. The responses of SS （no obvious effect） ,SP （significant depress effect） ,and Pro （significant stimulate effect） of Cs to SO2 stress were different. （3）On the whole, SO2 stress significantly increased the activities of antioxidant system enzymes （SOD,POD and CAT）, and the content of MDA in leaves of Eg and Cp. There were different responses of SOD, POD, CAT and MDA to SO2 stress of Se. However, under SO2 stress,the activities of antioxidant system enzyme activities and MDA content were overall declined significantly. （4）The maximum absorption of S element of Eg, Cp, Sc and Cs were 1. 17, 1.32,2.04,0.95 g . kg^-1 ,respectively. （5）The order of the comprehensive resistance calculated based on membership function was Cp〉Sc〉Eg〉Cs,which was consistent with the results of injury of leaves. This study suggests that the antioxidant system enzymes play a important role in resistance of SO2 stress for the four species of young trees and the extremely high level of basic POD activity in Cp may be one of the key mechanisms of resistance of SO2 stress.
出处
《西北植物学报》
CAS
CSCD
北大核心
2013年第12期2441-2450,共10页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31300522)
四川省教育厅重点项目(12ZA118
13ZA0246)
教育部高等学校博士学科点专项基金(20125103120018)
"十二五"科技支撑计划(2011BAC09B05)
关键词
SO2胁迫
幼树
生理响应
隶属函数
SO2 stress
young tree
resistance physiological response
membership function
