摘要
Topsis法可运用多指标对对象进行综合比较,本研究以此法综合比较不同成熟度的青川产北柴胡种子的萌发质量。观察4种成熟度青川产北柴胡种子的胚发育情况,测定种子可溶性糖、可溶性蛋白含量,分析种子吸水特性、萌发质量,荧光显微技术对种子进行香豆素相对定量分析,并用Topsis法综合评价各成熟度萌发质量。不同成熟度的种子在外观、千粒重、吸水性能、萌发特性、营养物质含量和解剖结构上均表现不同,各种成熟度的种子经过采后贮藏胚率均可发育到相近水平,随成熟度增加,种子千粒重增加,幼苗质量增强,香豆素含量也渐高,萌发速率和萌发率有所下降,Topsis法评价种子的综合质量结果为:绿熟>黄熟>褐熟>黑熟。青川北柴胡绿熟种子综合质量最佳,黄熟、褐熟次之,黑熟最差,生产上应及时采收,减少过熟种子的比例。
Topsis approach, a multi-indexes evaluation method, can be used for comprehensive comparison of objects. This approach was used to investigate Bupleurum chinense seeds＇ germination quality of different maturities from Qingchuan County. The anatomic structure of the seeds were observed with paraffin method, and their appearance was analyzed, and then the content of soluble sugar and soluble protein, thousand kernel weight （TKW）, as well as the seeds＇ germination indexes were determined. Seeds of different maturities differed in appearance, TKW, bibulous performance, germination characteristics, nutrients content and anatomical structure. After being post-harvested for 4 weeks, the embryo of seeds of different maturities can be developed into a similar level; with the increasing of the seeds＇ maturity, seeds＇ dry weight increased, seedling quality enhanced. Being analyzed with Topsis approach, the green seeds, which possessed a good germination characteristic, were recognized to be the superior group, followed by the yellow, the brown and the black. Black seeds＇ TKW and embryo rate were greater than the others, but their germination rate was low, these may attribute to the highest contents of coumarins, which both inhabited the embryo growing and germination. Green seeds＇ comprehensive quality is better than the others; followed by the yellow, and then the brown and the black. In terms of agricultural technique, it is better to harvest the seeds in time, hereby obtaining a low proportion of overripen seeds, which are inferior.
出处
《植物研究》
CAS
CSCD
北大核心
2014年第1期108-113,共6页
Bulletin of Botanical Research
基金
国家星火计划项目(2010GA810056)
四川省科技富民强县专项计划项目"青川县柴胡现代化科技产业基地建设"
四川省"十二五"育种攻关项目(2011NZ098-12-11)
