摘要
目的制备不同分子量壳寡糖,探究壳寡糖抗氧化、抗糖基化作用及其对高糖培养的红细胞内一氧化氮含量的影响,探究壳寡糖的分子量与生物活性的关系。方法纤维素酶降解法制备3种不同分子量的壳寡糖,依次命名为HD90、MD90、LD90。采用清除羟自由基实验,测定糖基化终级产物AGEs荧光强度和测定高糖培养的红细胞内NO含量增长率来研究不同分子量壳寡糖的作用活性。结果HD90、MD90、LD90对羟自由的清除率分别为89.02%、81.16%、65.38%(浓度4 mg/mL);对AGEs的抑制率分别为32.31%、31.14%、27.00%(浓度200μg/mL);对高糖培养的红细胞内NO含量增长率分别为333%、206%、65%(浓度5 mg/mL)。结论壳寡糖存在明显的抗氧化、抗糖基化作用,可以显著地提高高糖培养的红细胞内NO含量,同时证明壳寡糖的分子量与其生物活性有密切关系。
Objective To prepare chitooligosaccharides （COS） with different molecular weights and investigate the effect of COS on antioxidation, antiglycation and NO generation in erythrocyte. To further understand the relationship between molecular weight and bioactivity of COS. Methods Three kinds of COS were obtained by degradation with cellulase, they were named HD90, MD90, LD90, respectively. The assay of scavenging hydroxyl radical, assay of AGEs measurement and determination of NO content in erythrocyte were conducted to study the activities of COS. Results The scavenging activities of hydroxyl radical of HD90, MD90, LD90 （concentration of 4 mg/mL） were 89.02%, 81.16%, 65.38%, respectively; the inhibitory effect of HD90, MD90, LD90 （ concentration of 200 μg/mL） on AGEs formation were 32.31%, 31.14%, 27.00%, respectively; the rate of increasing NO content in erythrocyte of HD90, MD90, LD90 （concentration of 5 mg/mL） were 333%, 206%, 65%, respectively. Conclusion COS has obvious effect on antioxidation, antiglycation and NO promotion in erythrocyte. In addition, there is a close relationship between molecular weights and bioactivities of COS.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2013年第6期486-489,共4页
Journal of Harbin Medical University
基金
黑龙江省博士后科研启动基金(2010)
关键词
壳寡糖
抗氧化
抗糖基化
一氧化氮
生物活性
chitooligosaccharides
antioxidation
antiglycation
NO
bioactivity
