摘要
目的了解河南省肉鸡养殖和屠宰过程中沙门菌的污染状况，为食品安全风险评估提供数据支持。方法选择肉鸡产品销量占河南省市场60％以上的养殖场和屠宰场，于2010年6～10月份在肉鸡养殖环节采集成年鸡肛拭子、在屠宰环节采集鸡酮体按照GB4789．4－2010进行沙门菌定性或／和定量检测。结果共检测368只鸡肛拭子，沙门氏菌检出率8．7％；186份鸡酮体，检出率24．2％。鸡肛拭子检出率最高的月份是7、8月、鸡酮体检出率较高的月份分布在6、8月份；两类样本检出沙门菌血清型前3位一致，均为印第安纳、肠炎和哈达尔沙门菌。鸡酮体沙门菌污染量较低，91．1％在10MPN/g以下。检出沙门菌均具有invA基因。肉鸡胴体和活鸡肛拭子分离沙门菌均具有严重的多重耐药现象，活鸡肛拭子沙门菌较胴体沙门菌耐药严重。结论肉鸡沙门菌的污染取决于肉鸡携带沙门菌和养殖环境中沙门菌的污染状况，并通过屠宰过程使污染扩大化和复杂化。宰后酮体鸡沙门菌的含量不高，但是沙门菌的存在将对食品安全造成潜在危害。分离沙门菌菌株耐药现象严重，提示政府要加大监测力度，养殖部门要合理使用抗生素。
Objective To further understand the prevalence of Salmonella in broiler cultivation and slaughter processing in Henan Province to provide basic data for risk assessment of food safety. Methods Broiler farms and slaughter houses that broiler product sales volumes beyond sixty percent in Henan market were selected for sampling. Anal swabs of grown broile~ during raise and carcasses in slaughter processing were sampled for qualitative or/and quantitative Salmonella detection from June to October in 2010 ,according to the methods described in the National Standards of the People＇s Republic of China （GB/T 4789.4-2010）. Results The detection rates of Salmonella in 194 broilers anal swabs and 186 carcasses were 6.2 % and 24.2 %, respectively. Salmonella was most prevalent in July and August in anal swabs, and in June and August in carcasses. The three most prevalent Salmonella serotypes in two kinds of samples were all same, which were Salmonella Indiana, S. Enteritidis and S. hardar, respectively. The contamination rate in 91.1% of broils carcasses was below 10 MPN/g, and invA gene was detected in all Salmonella isolates. Serious multi-drug resistance was found in Salmonella isolates recovered from broilers carcasses and alive broilers anal swabs, and the isolates recovered in anal swabs were more resistant to antimicrobials than that from carcasses. ConclusionS Salmonella in broils were determined from the prevalence status of raise environment and alive animals, the slaughter processing enlarged Salmonella transmission and made the contamination more complication. Although the prevalence level of Salmonella in slaughtered broilers carcasses was low, however, it is do a potential hazard to food safety. Furthermore, theSalmonella isolates were resistant to antimicrobial reminded surveillance measures should be strengthened by our gov- ernment and antimicrobials should be used reasonably by animals raise farms.
出处
《河南预防医学杂志》
2014年第1期48-51,共4页
Henan Journal of Preventive Medicine
关键词
肉鸡
养殖
屠宰
沙门氏菌
定量
耐药性
食品安全
Broiler
Raise
Slaughter
Salmonella
Quantitative
Antimicrobial resistance
Food safety
