摘要
目的 建立和评价能准确检测食管鳞癌 (ESC)局部淋巴结 (LN)微小转移的RT PCR法。方法 取 2 3例ESC患者的局部LN共 10 4枚 ,将每枚LN均分为两等份 ,分别行病理学检查和CK19mRNA的表达分析。采用连续稀释试验评价方法的灵敏度。结果 本法的灵敏度为 10 -6～10 -4 μgESCRNA 1μg正常LNRNA。 2 2枚LN被病检法发现存在转移 ,RT PCR法发现其中 2 1枚为阳性。在余下的 82枚病检阴性LN中 ,RT PCR还发现 10枚存在微转移。结论 RT PCR法检测局部LN转移有助于ESC患者亚临床转移的早期诊断和治疗。
To set up means of reverse transcription-polymerase chain reaction(RT-PCR)which could be used to detect micrometastases in regional lymph nodes (LNs) of ESC patients and to compare the method with histological examination(HE).Methods Serial dilution study using ESC tissue was performed to detect the sensitivity of CK 19 RT-PCR assay.The regional LNs( n =104) were obtained from 23 ESC patientswho underwent operation.Each LN was cut into halvers.One half of each LN was subjected to HE.The other half was subjected to RT-PCK.Results The sensitivity of this assay was 10 -6 ～10 -4 μg ESC RNA/1μg normal LN RNA.Twnnty-two of 104 LNs were histologically involved by carcinoma cells and 21 of these yielded the expected product by RT-PCK.Of the 82 Lns which were diagosed to be devoid of metastases by HE,ten Lns were found to express CK 19 mRNA.Conclusion The assay of RT-PCK may lead to an earlier diagnosis and treatment of ESC patients with subclinical LNs metastasis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2000年第5期347-349,共3页
Cancer Research on Prevention and Treatment
关键词
食管癌
食管鳞癌
淋巴结微小转移
诊断
RT-PCR
Esophageal carcinoma
Lymph nodes
Metastases
Diagnosis
keratin
Polymerase chain reaction
