摘要
目的优选佛手的最佳醇提工艺,以期对大生产提供试验依据。方法以佛手代表性成分———橙皮苷为考察指标,对HPLC外标法测定橙皮苷含量进行了方法学考察;比较了20%、30%、40%、50%、60%、70%、80%等7种不同浓度的乙醇以及浸渍提取、超声提取、加热回流提取等3种不同提取方法对佛手提取效果的影响;然后采用L9(34)正交试验法,考察乙醇用量、提取时间及提取次数对提取效果的影响。结果提取次数与乙醇用量对考察指标具有显著性影响。佛手的最佳醇提工艺为加40倍药材量的30%乙醇,加热回流提取3次,每次0.5 h。结论优化后的橙皮苷含量测定方法稳定,准确可靠,为佛手质量的评价提供了依据。
Objective To optimize the extraction technology of Citrus lnedica L. var. sarcodactylis Swingle with alcohol. Methods The extraction effects were investigated by different concentrations （20% ,30% ,40% ,50% ,60% ,70% ,80% ） of ethanol,as well as diver- sified extraction processes （such as immersion extraction,ultrasonic extraction, reflLtxing extraction） , additionally,the orthogonal test of L~ （34） was employed to study the effect of quantities of ethanol,extraction time and extraction fi-equeneies and the results were deter- mined by HPLC with Hesperidin of Citrus lnediea L. vat＇. sareodaetylis Swingle as the observation index. Results The extraction fi＇e- queney and quantity of ethanol played a prominent role on the extraction effect,while the optilnal condition was 40-fold 30% （v/v） eth- anol the amount of medicinal material at a reflttx for 30 luln and extracted three times. Conclusion The optimized extraction technology shows stabilization and accuracy,which can be used to evaluate Citrus lnediea L. var. sareodactylis Swingle.
出处
《安徽医药》
CAS
2014年第1期26-29,共4页
Anhui Medical and Pharmaceutical Journal
关键词
佛手
醇提工艺
正交试验
橙皮苷
高效液相法
Citrus lnedica L. vat＇. s arcodactylis Swingle
extraction technology with alcohol
orthogonal test
hesperidin
HPLC
