摘要
目的了解上海地区大流行副溶血性弧菌(VP)大流行菌株血清型分布及分子特征。方法对2010—2012年分离自腹泻患者和食品中的VP菌株进行血清分型,以GS-PCR辨别大流行株,以PCR检测菌株的毒力基因tdh、trh和大流行株分子标识f237噬菌体orf8基因,以PFGE分型来分析菌株间的遗传关系。结果 1 136株VP可分为52个血清型(群),其中64.5%的菌株GS-PCR阳性,判定为大流行菌株,其血清型有11种,主要集中于O3∶K6(76.8%)、O4∶K68(9.4%)、O1∶K25(6.8%)、O1∶K36(4.5%)4种血清型。相对于非流行菌株,O10∶K60、O3∶K3、O1∶K33三种血清型的菌株其PFGE图谱与已报道的大流行株更为接近,为新检测到的大流行血清型变种。大流行产毒株中仅能检测到tdh基因,未检测到trh基因,94.3%的大流行菌株携带噬菌体f237的orf8基因,但有5.7%的大流行株orf8基因缺失。相同血清型的大流行菌株其PFGE图谱存在差异,且orf8基因的携带与否不能以PFGE进行区分。结论上海地区VP大流行菌株血清型相对集中,且不断有新的血清型变种出现。从PFGE图谱的差异和orf8基因的携带与否上来看,大流行菌株仍在演变。
Objective The main purpose of this research was to describe the distribution of V. parahaemolyticus serotypes isolated from diarrhea cases and food in Shanghai and the molecular characteristics of pandemic strains. Methods GS-PCR was used to recognize pandemic strains, tdh gene, trh gene and orf8 gene of phage f237 were detected by PCR after serotyping. PFGE was used to analyze the genetic relationship among isolates. Results 1 136 isolates were divided into 52 serotypes （groups）. 64. 5% of all isolates were GS-PCR positivewhich wasthe pandemic strains. 11 serotypes were found in pandemic strains in Shanghai, among which O3：K6 （76. 8% ） , O4：K68 （9.4%）, O1：K25 （6. 8% ）, and O1：K36 （4. 5% ） were the main serotypes. Pandemic strains with serotypes of O10： K60, 03：K3 and O1：K33 were first reported, and their PFGE patterns were closer to the reported pandemic strains. Most-pandemic strains only had tdh gene, and no trh gene positive. Except 5.7% strains, most pandemic strains were positive for orfSgene. PFGE patterns were different among pandemic strains with same serotypes, and isolates with or without orf8 gene could not be distinguished by PFGE. Conclusion New serovariants of pandemic were emerging, and the results of PFGE and orf8 gene detection suggested there was gene mutation amongpandemic strains.
出处
《中国食品卫生杂志》
北大核心
2014年第1期5-9,共5页
Chinese Journal of Food Hygiene
基金
副溶血性弧菌流行菌型演变趋势及毒力因子进化研究(2010Y105)
上海市公共卫生重点学科-卫生微生物学(12GWZX0801)
