摘要
寄生虫可引起食源性疾病,已成为影响食品安全的重要因素之一。传统的病原学方法常受制于技术人员的技术熟练程度和鉴别能力,检测效率不高。因此,快速准确的检测技术成为食品寄生虫诊断领域发展的一大趋势。本文对目前先进的快速检测技术,如聚合酶链式反应(PCR)、经化学试剂特殊处理的棉纤维卡片(FTA)、环介导等温扩增(LAMP)、基因芯片(DNA chip)以及胶体金免疫层析(GICA)等技术在食品寄生虫检测中的应用和研究进展进行综述。
Food-borne diseases caused by parasites have become one of the important factors affecting the food safety. Traditional etiology methods were often subject to the technical proficiency and identification ability of teehinician. In addition, the detection efficiency can not be satisfied with the actual needs. Therefore, rapid and accurate detection is a trend to food-borne parasite diagnosis development. The research progress and its application of foodborne parasite detection with some modern detection techniques, such as polymerase chain reaction （PCR）, flinders technology associates （FTA）, loop-mediated isothermal amplification （LAMP）, microarray （DNA chip） and colloidal gold immune chromato-grapby （GICA） technology, including their mechanisms, advantages and disadvantages is reviewed in this paper.
出处
《中国食品卫生杂志》
北大核心
2014年第1期95-100,共6页
Chinese Journal of Food Hygiene
