摘要
目的:观察胃和冲剂Ⅱ号对CAG(Chronic Atrophic Gastritis,CAG)大鼠胃黏膜EGF和EGFR表达的影响,探讨胃和冲剂Ⅱ号治疗CAG的作用机制。方法:将72只雄性Wistar大鼠随机分为正常对照组12只和造模组60只。除正常对照组外,其余各组大鼠均使用综合造模法成功复制CAG动物模型。然后将造模组分为5组,分别为模型组,维酶素对照组,胃和冲剂Ⅱ号低、中、高剂量组。对各组大鼠施加相应治疗因素60 d后,取各大鼠相同部位全层胃壁组织,采用免疫组化法分别检测胃黏膜组织的EGF和EGFR的表达水平。结果:EGF在胃和冲剂Ⅱ号高剂量组中的表达值为(1.25±0.16),低于CAG大鼠模型组的表达值(2.98±0.15),差异有统计学意义(P<0.01);胃和冲剂Ⅱ号高剂量组EGFR的表达值为(1.636±0.809),低于模型组的表达值(3.300±1.337),差异有统计学意义(P<0.01)。结论:胃和冲剂Ⅱ号可能通过降低胃黏膜组织的EGF和EGFR的表达,加快胃黏膜细胞的修复和抑制过度增殖,从而对CAG起到治疗作用。
Objective：To explore the treatment effect of Weihe granule II on CAG by analyzing the expression level of EGF and EGFR in gastric mucosal of model rats of CAG treated with Weihe granule II .Methods：Seventy-two male Wistar rats were randomly divided into two groups, control group （n=12） and model group （n=60）.The rat model of CAG in model group was created using comprehensive modeling method.Then, all rats in model group were assigned into five sub-groups, namely model sub-group, Vitacoenzyme control sub-group, low-dose sub-group, mediate-dose sub-group and high-dose Weihe granule II sub-groups.After all rats in each group were sub-jected to the corresponding treatments for 60 days, the expression level of EGF and EGFR in the same gastric tissue of each rat were be examined and compared with other groups .Results：The expression of EGF in the high-dose Weihe granule II sub-group （1.25 ±0.16） was lower than that in model sub-group （2.98 ±0.15）, （P〈0.01）;the expression of EGFR, in the high-dose Weihe granule II sub-group （1.636 ±0.809） was lower than that in model sub-group （3.300 ±1.337） （P〈0.01）.Conclusion：Weihe granule II can treat CAG by decreasing the expression levels of EGF and EGFR in gastric mucosal to repair the gastric mucosal cells and inhibit the excessive proliferation for cancer prevention .
出处
《世界中医药》
CAS
2014年第1期81-83,共3页
World Chinese Medicine
基金
内蒙古卫生厅医疗卫生科研计划项目(编号:2010022)
关键词
胃和冲剂Ⅱ号
慢性萎缩性胃炎
EGF
EGFR
Weihe granule
Chronic atrophic gastritis
EGF
EGFR
