摘要
目的观察不同海拔大鼠血清肿瘤坏死因子α（TNF-α）、白细胞介素（IL-10）在重症急性胰腺炎（SAP）中的表达水平及其与胰腺组织病理变化之间的关系,为临床诊断和治疗SAP提供实验依据.方法72只SPF级健康雄性Wistar大鼠随机分为海拔1500米（L）组、海拔3300米（M）组、海拔4300米（H）组.每组随机分成假手术组、造模6h、12h、24h组.造模组行胰胆管逆行注射5％牛磺胆酸钠建立SAP模型,假手术组仅打开腹腔翻动胰腺数次.造模组分别在造模后第6、12、24小时分批随机处死大鼠,假手术组在6h处死大鼠.所有大鼠处死后均采集血清及胰腺组织.ELISA法测定血清TNF-α、IL-10含量;胰腺损害程度采用病理评分评价.结果与假手术组比较,同一海拔内造模组各时点胰腺病理评分、IL-10、TNF-α差异均有统计学意义（P＜0.05）.同一海拔内造模组胰腺病理评分、IL-10的表达随着时点的延长而增加,TNF-α随时点的延长而减少.造模组同一时点L组与M组、L组与H组比较,胰腺病理评分、TNF-α和IL-10差异有统计学意义（P＜0.05）,H组与M组比较差异无统计学意义（P＞0.05）.IL-10与胰腺病理评分呈正相关,TNF-α与胰腺病理评分呈负相关.结论在SAP时,TNF-α水平随海拔增高而增高,但随时点延长显著降低,IL-10水平随海拔增高和时间延长而持续上升,提示二者在不同时段对SAP的发生发展起着重要作用.
Objective To observed the expression of serum TNF-α and IL-10 in rats with severe acute pancreatitis （SAP） at different altitudes,and to explore the relationships between TNF-α and IL-10,the pathological changes of the pancreas,and the experimental basis for clinical diagnosis and treatment of SAP.Methods 72 specific pathogen free （SPF） Wistar male rats were divided randomly into three groups：1 500 meters altitude （group L）,3 300 meters altitude （group M）,and 4 300 meters altitude （group H）.These three groups were then each divided randomly into four subgroups：control （group n）,6 hours after pancreatitis （group p 6 h）,12 hours after pancreatitis （group p 12 h）,and 24 hours after pancreatitis （group p 24 h）.Pancreatitis was induced by intraductal administration of 5% sodium taurocholate hydrate （NaTc）.The rats were killed at 6,12,and 24 hours after NaTc injection in groups p.The group n rats were killed after 6 hours of pancreas observation.Blood samples and pancreatic tissues were collected post mortem and enzyme-linked immunosorbent assay （ELISA） measured serum TNF-α and IL-10.Results Compared with the control （group n）,histopathological scores,IL-10,and TNF-α in the same altitude had a significant difference （P 〈 0.05） in group p at each time point.In the same altitude of group p,histopathological scores and IL-10 were increased with time elapsed （P 〈 0.05）,while TNF-α was decreased with time elapsed （P 〈 0.05）.There was a significant difference between group Mp and Lp in histopathological scores,IL-10,and TNF-α （P 〈 0.05）,and the same result between group Hp and Lp （P 〈 0.05）,but there was no significant difference between group Hp and Mp （P 〈 0.05）.Meanwhile,IL-10 had a positive relationship with histopathological score,but TNF-α had a negative relationship with histopathological score.Conclusions The level of TNF-α increased with increasing altitude but significantly reduced with elapsed time.The level of IL-10 increased with both increasing altitude elapsed time.These results suggested that TNF-o and IL-10 might play a important role at different times in severe acute pancreatitis.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2014年第1期56-59,共4页
Chinese Journal of Hepatobiliary Surgery
