摘要
苦瓜作为药食同源的植物,含有许多活性成分,其中核糖体失活蛋白MAP30是近年来研究较多的一种。根据Genbank中MAP30序列,采用PCR技术,从苦瓜基因组DNA中扩增出该基因片段,将其克隆到原核表达载体pET-28a上,构建重组质粒pET-MAP30,经克隆载体转化菌液PCR鉴定,结果显示成功构建MAP30的原核表达载体。
MAP30, extracted from Momordica charantia （bitter melon） was paid more attention in the recent years. The sequence of the gene, which encodes the MAP30 protein had been reported in GeenBank, it was amplified by PCR from the total DNA of bitter melon. The PCR product containing MAP30 gene was inserted into pET-28a expressive vector. The PCR results of liquid culture containing cloning vector tranfected bacteia demonstrated that the construction was successful.
出处
《武夷学院学报》
2013年第5期24-27,共4页
Journal of Wuyi University
关键词
苦瓜
MAP30
原核表达载体
bitter melon
MAP30
prokaryotic expressing vector
