摘要
目的探讨食物过敏(FA)患儿特异性抗体和细胞免疫因子变化特点及氨基酸乳品联合益生菌的干预效应。方法对临床疑似为FA的患儿(50例),通过食物回避试验,以及酶联免疫吸附法(ELISA)检测特异性抗体,分为单一过敏组15例、多种过敏组15例和非FA组20例,以及根据抗体阳性情况分为IgG阳性组20例、IgE阳性组10例和非FA组20例。采用流式细胞学检测淋巴细胞亚群以及CD23指标水平,并做对照研究。并且对26例牛奶蛋白过敏的患儿随机分两组,通过应用氨基酸配方奶粉以及联合益生菌两种治疗方案,治疗前后对照其免疫指标变化,对结果进行统计学分析。结果对于FA患儿,单一过敏组和多种过敏组相对非FA组,CD+3CD+8的淋巴细胞百分比水平明显降低,CD-3/CD+19明显升高,IgE阳性组CD23较其他组升高明显。应用氨基酸配方奶粉以及联合益生菌两种方案干预,可以降低患儿CD+3CD+4/CD+3CD+8与CD-3/CD+19细胞的水平。结论 FA患儿体内的部分免疫指标有所改变,应用氨基酸配方奶粉或是联合益生菌干预下,可改善牛奶过敏患儿的临床症状以及调节机体免疫状态。
Objective To study the characteristics of specific antibodies and cell immune factors change in children with FA and a- mino acid formula plus probiotics intervention effects of studies. Methods 50 children with FA was divided into The single group （ n = 15 ）, multiple group （ n = 15 ） and negative group （ n = 20） ; IgG （ n = 20）, IgE positive group （ n = 20） and negative group （ n = 20 ）, according to the test of specific antibodies with ELISA. Then the differences were analyzed. In the meanwhile, 26 children with milk allergy was divid- ed into two groups randomly,and formula with amino acid and probiotics were separately used in the two groups. Finally, the differences of immune targets and statistical differences were analyzed. Results To the children with FA, there was significant difference between the sin- gle group and multiple group, the same as the percentages of CD3 ＋ CD8 ＋ and CD3 -/CD19 ＋ . CD23 in the IgE positive group significantly higher. Formula with amino acid or probiotics within 6 months can better improve the CD3 ＋ CD8 ＋ and CD3 -/CD19＋ of the children. Conclu- sions The immune targets in the body of the children with FA had changed. The use of formula with amino acid or probiotics can better im- prove the clinical symptoms and the bodyg immunological function of the children with milk allergy.
出处
《安徽医学》
2014年第1期75-78,共4页
Anhui Medical Journal
