摘要
目的:探讨儿童化学发光免疫分析中标本量过少时,用怎样的稀释介质以及稀释方法解决这个问题,分析不同的稀释介质产生的基质效应。方法:本实验以儿童常规筛查检测项目血清三碘甲状腺原氨酸(TT3)为例,选取血清样本30例,分别使用美国贝克曼库尔特公司UniCel DXI800 Access化学发光免疫分析仪测试药盒三碘甲状腺原氨酸(TT3)配套TT3定标液S0(简称TT3S0)、0.9%氯化钠、医用蒸馏水作为稀释介质进行手工2倍(1:1)及4倍(1:3)后测定。计算稀释测定结果与原始数据的差异,观察基质效应对检测结果的影响。寻找实际工作中可运用的稀释介质和稀释倍数。结果:不同稀释组与原始值之间比较,TT3S02倍(t=0.7937,P>0.05)、4倍稀释(t=-0.2503,P>0.05),以及蒸馏水2倍稀释(t=-0.2845,P>0.05)差异均无统计学意义。而0.9%氯化钠2倍(t=-6.4686,P<0.05)、4倍稀释(t=-3.9842,P<0.05),以及医用蒸馏水4倍稀释(t=-1.9957,P<0.05)差异有统计学意义。结论:三碘甲状腺原氨酸TT3S0定标液2倍,4倍稀释方法及医用蒸馏水2倍稀释方法均可以得到较为满意的结果。三碘甲状腺原氨酸(TT3)配套TT3定标液S0可用于标本2倍及4倍稀释。当定标液获取困难,蒸馏水2倍稀释同样可以运用。
Objective： To address the problem of undersampling in child chemiluminescene immunoassay by investigating the influence of dilution medium and method on detection of serum triiodothyronine（Total T3） concentration. Methods： Serum triiodothyron- ine （Total T3） concentration, a routinely screened indicator in children physical examinations, was tested. Serum samples were collected from 30 children. UniCel DXI800 appliance produced by Beckman Coulter Inc was used to test the concentration of serum triiodothyronine as diluted two and four times by Access Total T3 Calibrators SO （TT3S0）, 0.9 % sodium chloride injection, and sterile water for injection respectively. By comparing the concentration test results before and after dilution, the matrix effect on test results was investigated. Based on the experimental results, the workable diluents and dilution multiples can be identified to be applied in medical tests. Results： Paired t-tests between before-and aider-dilution groups showed that the difference is statistically insignificant in samples diluted by TT3S0 two times （t=-0.7937, P〉0.05）, TT3S0 four times dilution （t=- -0.2503, P〉0.05）, and sterile water two times dilution （t=- -0.2845, P〉0.05）. The difference is statistically significant in samples diluted by 0.9 %sodium chloride injection two times （t= -6.4686, P〈0.05）, 0.9 %sodium chloride injection four times （t= -3.9842, P〈0.05） and sterile water four times （t=- -1.9957, P〈0.05）. Conclusion： TT3S0 two times and four times dilution, as well as sterile water two times dilution can work as satisfactory dilution method.TT3S0 can be diluted 2 or 4 times to be applied as dilution medium. When it is difficult to get access to the above dilution medium, sterile water two times dilution can also be used.
出处
《现代生物医学进展》
CAS
2014年第1期114-116,107,共4页
Progress in Modern Biomedicine
关键词
稀释
化学发光免疫分析
儿童
Dilution
Chemiluminescent Immunoassay
Children
