摘要
目的本实验旨在初步探讨A/Shanghai/4664T(H7N9)和A/Puerto Rico/8/34(H1N1)病毒感染BALB/c小鼠的发病特点,为H7N9致病机制与防治的研究提供数据支持。方法将同等剂量(5×103TCID50)流感病毒滴鼻感染BALB/c小鼠,分析其在感染后体重、肺指数、病毒载量和肺组织病理的变化情况。结果 H1N1PR8感染组和H7N9感染组小鼠在7 d内体重均持续减低,H1N1 PR8组较H7N9组降低更为明显;在感染后3 d H7N9组与PBS组肺指数无显著差异(P>0.05),但感染后7d H7N9组与PBS组相比显著增高(P<0.05);H1N1PR8组比H7N9组感染后3 d肺指数显著增高(P<0.05),但感染后第7天无显著差异(P>0.05)。H1N1 PR8组和H7N9组病毒载量在感染后第3天均显著升高,但两组无显著差异;第7天两组病毒载量均有所降低,但H7N9组病毒载量显著高于H1N1 PR8组(P<0.05)。H1N1 PR8在感染后3 d主要病理变化为炎症细胞浸润和水肿,而H7N9组主要轻度炎症细胞浸润;在感染后7 dH1N1 PR8组可见大量炎症细胞浸润,出现大面积肺水肿;H7N9组表现为大量炎症细胞浸润。结论 H7N9和H1N1 PR8感染BALB/c小鼠均可发病,但发病特点有所差异,H7N9病毒对鼠的适应性比H1N1 PR8差。
Objective To compare clinical characteristics of BALB/c mice infected by H7N9 （A/Shanghai/ 4664T） and H1 N1 PR8 （A/Puerto Rico/8/34） virus for providing clues for H7N9 virus pathogenic mechanism researches.Method Each BALB/c mouse was inoculated by 5× 10^3 TCID50 H7N9 or H1N1 PR8 virus or PBS after anesthetize. The bodyweight change, lung index, viral load and pulmonary pathology were monitored and analyzed. Result BALB/c mice infected with H1N1 PR8 and H7N9 showed clinical characteristic differences. In terms of bod yweight change, both H1 N 1 PR8 group and H7N9 group were reduced gradually within 7 days post infection （d. p. i） , though the bodyweight of H1N1 PR8 group decreased more rapidly than that of H7N9 group. The lung viral loads of H1 N1 PR8 group and H7N9 reached peak at 3 d. p. i, and reduced to very low level at 7 d. p. i. Nevertheless, though the H1N1 PR8 group and HTN9 have no significant difference at 3 d. p. i, H7N9 group showed a significantly higher level of viral loads than that of H1N1 PR8 group at 7 d. p. i （P 〈 0.05 ）. For lung pathological aspects, the major lesions of H1N1 PR8 group were infiltration of inflammatory cells and edema; H7N9 group showed mild inflammatory cell infiltration at 3 d. p. i. Subsequently at 7 d. p. i, the mainly lesions of HI N1 PR8 group were abundant of inflammatory ceils infiltration with a massive pulmonary edema; H7N9 group showed a large number of inflammatory cells infiltrating in the lung. Conclusion BALB/c mice infected by H7N9 and H1 N1 PR8 showed differences in pathogenic characteristics. H7N9 virus showed less mouse adaptability than the H1N1 PR8 virus. We need to consider the impact of virus titre on follow-up evaluation studies for the efficacy of potential H7N9 therapy drug and vaccine.
出处
《中国比较医学杂志》
CAS
2014年第1期40-44,I0010,共6页
Chinese Journal of Comparative Medicine
基金
上海市科委资助项目(12140903100)
科技部人感染H7N9禽流感应急防控研究专项(KJYJ-2013-01-04)
上海市公共卫生临床中心H7N9应急项目(201316)
