摘要
目的探讨不同抗原修复法对免疫组织化学法检测子宫颈鳞状上皮病变中p16和cyclin D1表达的影响。方法采用45例临床常规检测的子宫颈鳞状上皮病变组织及p16和cyclin D1特异性抗体。探讨p16和cyclin D1 IHC检测最佳的抗原修复方法。抗原修复法分为4组:A、B组采用EDTA修复液,B组使用高压锅1.5分钟;C、D组采用柠檬酸盐缓冲液(citrate buffer,CB)和高压锅1.5分钟和3分钟。结果采用Wilcoxon两组等级资料的非参数检验比较,A组与B组、C组与D组两者之间p16检测差异无统计学意义(P>0.05);B组与C组在CINⅠ级和Ⅱ级病变中p16检测差异均有统计学意义(P=0.001;P=0.028)。两液修复后,cyclin D1在4组抗原修复方法间的表达阳性范围差异不大,其表达部位均在子宫颈基底细胞核内,少数子宫颈柱状上皮细胞也有表达,在子宫颈各级病变靠近基底细胞区域散在表达,但用EDTA液在表达强度上较CB液明显,B组最强,数量有所增加,背景染色深,胞质普遍着色。A组阳性信号稍减弱,颜色亮丽,对比明显,偶尔出现胞质着色。结论切片用CB液修复,p16在子宫颈病变中的表达比较符合病情演变。
Objective To compare the effect of different antigen retrieval methods of immunohistochemistry in detection of p16 and cyclin D1 in cervical squamous epithelial lesions. Methods Expression of p16 and cyclin D1 was detected by immunohistochemistry in 45 tissue specimens of cervical squamous epithelial lesions. Four antigen retrieval methods were applied in immunohistochemistl% Groups A and B use EDTA as repairing fluid, with B using pressure cooker for 1.5 rain ;Groups C and D use citrate buffer （CB） and pressure cooker for 1.5 rain and 3 rain respectively. Wilcoxon nonparametric test was used for comparison. Results There was no significant difference in p16 detection between methods A and B or methods C and D（P 〉0.05）. There were significant differences in p16 expression in lesions of both CIN class ] and [[ between methods B and C （P = 0. 001 ;P = 0. 028 ）. There was no difference in the expression of cyclin D1 among 4 antigen repair methods. The positive staining was located in the cervical basal nuclei, a small number of cervical columnar epithelial cells and all levels of cervical lesions near the basal cell area; but the express strength of cyclin D1 when using EDTA was stronger than that using CB. Staining was the strongest in method B with intense background staining and generally colored cytoplasm. In method A positive signal was slightly diminished, contrast was marked and endochylema was occasionally stained. Conclusion hnmunohistochemical staining of p16 in cervical lesions by using CB for antigen retrieval may reflect the evolution of lesions more clearly.
出处
《实用肿瘤杂志》
CAS
2014年第1期80-82,共3页
Journal of Practical Oncology
