摘要
研究了禁食及再投喂对瓦氏黄颡鱼(Pelteobagrus vachelli)幼鱼(体质量平均为(3.12±0.16)g)皮质醇及糖代谢的影响。试验分成5组,分别为对照组(G1)正常投喂、禁食1 d(G2)、禁食7 d(G3)、禁食14 d(G4)、禁食21 d(G5)后再恢复投喂。选取样品分析测定了瓦氏黄颡鱼幼鱼禁食和再投喂后皮质醇水平、肝指数、血糖浓度、肝糖原及肌糖原含量。结果表明:G2组幼鱼皮质醇水平与对照组无显著性差异(P>0.05),而G3、G4、G5组则极显著升高(P<0.01),恢复投喂4 d后幼鱼皮质醇水平恢复至对照组水平。禁食后幼鱼的肝指数随禁食时间的增加而降低,恢复投喂后肝指数逐渐恢复至对照组水平。G2、G3、G4组幼鱼的血糖浓度在禁食后与对照组无显著性差异(P>0.05),而G5组幼鱼在禁食21 d后的血糖浓度显著下降(P<0.05)。G3、G4、G5组幼鱼在禁食后肝糖原含量显著降低(P<0.05),在恢复投喂的过程中G3、G4、G5组幼鱼的肝糖原含量升至对照组的约1.1到3.7倍。在整个试验过程中所有组别幼鱼的肌糖原含量均无显著变化(P>0.05)。
The effects of fasting and refeeding on cortisol and metabolism of glucose in juveniles of Pelteobagrus Vachelli （body weight： （3.12 ＆#177;0.16） g were studied.The fish were distributed in five experimental groups .The first group was considered as the control and was fed normally; the others were fasted for 1, 7, 14, 21 d and re-fed.The concentration of cortisol, HSI, blood glucose and liver and muscle glycogen in P.vachell juveniles were determined during starvation and refeeding.The results were as follows： after fasted, the cortisol levels of G2 was not significantly different from that of the control （ P＆gt;0.05 ） .While the cortisol levels of G 3 , G4 , G5 significantly increased by compared with the control （ P＆lt;0.01 ） , and returned to basal level after 4 days of refeeding .The HSI of juveniles decreased with the increase of fasting periods and returned to control level after refeeding .Blood glucose values in juveniles of G 2, G3, and G4 remained un-charged during fasting with no significantly difference （P＆gt;0.05） from the control.At the end of 21-day fasting period, blood glucose values in juveniles of G 5 decreased significantly （ P＆lt;0.05 ） , but 2 days after refeeding , liver glycogen in-creased with values ranging from 1.1~3.7 times those of control concentrations .In contrast, no starvation effect was veri-fied in the muscle glycogen （ P＆gt;0.05 ） .
出处
《淡水渔业》
CSCD
北大核心
2014年第1期36-40,共5页
Freshwater Fisheries
基金
中央高校基本科研业务费专项资金(XDJK2012C091)
中国长江三峡集团公司项目
