摘要
摘要：将一端标记具有可逆电化学行为的电活性基团二茂铁、另一端标记巯基的三磷酸腺苷（ATP）适体SS—DNA组装到薄膜金电极上，构筑了一种可灵敏检测ATP浓度的交流阻抗型适体传感器。首先研究了适体传感器的自组装方法，然后利用交流阻抗法研究了适体传感器在铁氰化钾（K3Fe（CN）6）和亚铁氰化钾（K4Fe（CN）6）混合溶液中的电极表面电子传递阻抗和被测物ATP浓度之间的关系，最后对自组装的阻抗型适体传感器的电化学特性进行了分析。实验表明：利用自纽装的适体传感器检测ATP浓度时，适体传感器检出限低，线性检测范围宽，重复性好，其中检出限为0．5μmol／L，线性检测范围为0．5～100μmol／L，线性度为0．9989，连续5次测量的相对标准差为1．5％。
Abstract： The electroactive group ferrocene labeled with reversible electrochemical behavior at the end and the aptamer ss-DNA of the adenosine triphosphate （ATP） labeled with sulfydryl at the other end were self-assembled on the thin-film gold electrode, and an AC impedance aptasen- sor was fabricated to measure sensitively the concentration of the ATP. Firstly, the self-assembly method of the aptasensor was researched. Then the relationship between the concentration of the ATP and the electron transfer impedance on the surface of the electrod in the of K3Fe（CN）6 and K4Fe（CN）6 mixed solution was studied by AC impedance method. Finally, the electrochemical characteristics of the self-assembled impedance aptasensor were analyzed. The experiments indi- cate that the aptasensor is of low detection limit, wide linear detecting range and good repeatabi- lity when the concentration of the ATP is measured with the self-assembled aptasensor. The detection limit is 0.5μmol/L, linear detecting range is 0.5 - 100 μmol/L, the linearity is 0. 998 9 and the relative standard deviation of continuous quintic measurements is 1.5 %.
出处
《微纳电子技术》
CAS
北大核心
2014年第2期105-109,114,共6页
Micronanoelectronic Technology
基金
国家自然科学基金资助项目(61002007)
河南省科技创新人才计划资助项目(124100510001)
