摘要
大气中CO2浓度持续升高和全球气候变暖是亟待解决的重大环境问题。自养微生物在环境中广泛分布,能直接参与CO2的同化,因此研究自养微生物同化CO2的分子生态学机制具有重大的科学意义。以往对自养微生物的研究多针对基因组DNA,从DNA水平揭示了不同生态系统中碳同化自养微生物的种群结构和多样性,但这些微生物在生态系统中的具体功能有待进一步的研究。近年来,随着转录组学研究技术和稳定同位素探针技术(SIP)的发展,自养微生物同化CO2的生态机理研究不断深入,这些研究明确揭示了碳同化自养微生物是河流、湖泊和海洋生态系统中CO2固定作用的驱动者,并新发现了一些具有CO2同化功能的微生物群落。基于国内外有关研究进展,从DNA和RNA水平上对自养微生物同化CO2的分子机理以及稳定同位素探针技术(SIP)在碳同化微生物研究中的应用进行了分析和总结,初步展望了RNA-SIP技术在陆地生态系统碳同化微生物分子生态学研究中的前景。同时,探讨了陆地生态系统同化碳的转化和稳定性机理,以期为深入了解生态系统碳循环过程和应对气候变化提供理论依据。
The increase in atmospheric carbon dioxide （CO2 ） concentration and global climate change are the most important environmental challenges we faced. Autotrophic bacteria, with the capability of CO2 assimilation, are widespread in diverse environments, thus studies on molecular mechanisms of autotrophic bacteria CO2 fixation are of great scientific importance. Previous researches were mainly based on genomie DNA, revealing the diversity and composition of CO2 fixation microorganism in diverse ecological systems. However, the functional implication of these autotrophies is still unknown. Transcriptomics technique and stable isotope probe technique （SIP） are successfully employed in recent studies concerningthe ecological function of CO2 fixation microorganism, providing unequivocal evidence for CO2 assimilation by autotrophic microorganisms in complex aquatic environments and identifying new autotrophic bacteria capable of CO2 assimilation. Therefore, the microbial mechanisms of CO2 fixation by autotrophic mciroorganism based on SIP are summarized and the methodological considerations of RNA-SIP for molecular ecology of carbon fixation in agricultural ecosystems are presented. The translocation and stability of terrestrial ecosystem carbon cycle assimilated carbon were also discussed. This paper will offer the theoretical basis of and response to climate change
出处
《生态学报》
CAS
CSCD
北大核心
2014年第3期701-709,共9页
Acta Ecologica Sinica
基金
国家自然科学基金资助项目(41271279
41090283)
湖南省自然科学基金资助项目(11JJ4030)
中国科学院青年创新促进会会员资助项目(2012-2015)
中国科学院亚热带农业生态过程重点实验室开放基金资助项目(2010-2012)
