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吖啶橙-罗丹明6G能量转移荧光法测定痕量磷 被引量:13

The Energy Transfer Fluorescence Method for Determination of Trace Phosphorous with Acridine Orange-Rhodamine 6G
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摘要 在λex/λem=45 0 / 5 5 6nm ,十二烷基苯磺酸钠 (DBS)存在下 ,吖啶橙 罗丹明 6G能够发生有效能量转移 ,使罗丹明 6G荧光大大增强 ;酸性条件下 ,正磷酸根与钼酸盐反应生成磷钼酸 ,磷钼酸与R6G形成离子缔合物 ,使R6G的荧光猝灭。利用吖啶橙 罗丹明 6G能量转移荧光法测定痕量磷 ,提高了测定磷的灵敏度和选择性。磷含量在 0 0 5~ 0 .70 μg/L范围内与罗丹明 6G的荧光猝灭程度呈良好的线性关系。最低检出限为 5ng/L ;回收率为 95 %~ 110 % ;测定 0 .5 0 μg/L磷溶液 ;相对标准偏差为 6 %。该法用于水样及土壤中活性磷的测定 。 It was found that at the condition of lambda (ex)/lambda (em) = 450/556 nm, in the dodecyl benzene sodium sulfonate solution, the effective energy transfer could occur between AO and R6G. The fluorescence intensity of R6G was diminished by molybdophosphate which was formed by the reaction between orthophosphate and molybdate in hydrochloric acid medium. The detection limit of this method was 5 ng/L. The range of determination for phosphorous was 0.05 similar to 0.70 mug/L. The relative standard deviation for the determination of 0.5 mug/L phosphorous was 6% (n = 13). The recoveries for the addition of 0.2 similar to 0.4 mug/L phosphorous were 95% similar to 110%. The method has been applied to the determination of phosphorous in water samples and soil samples with satisfactory reuslts.
出处 《分析化学》 SCIE EI CAS CSCD 北大核心 2001年第1期42-44,共3页 Chinese Journal of Analytical Chemistry
关键词 吖啶橙 罗丹明6G 能量转移 荧光光度法 土壤 痕量分析 phosphorous acridine orange rhodamine 6G energy transfer fluorescence
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  • 1王明雄,1988年
  • 2王筱敏,光谱学与光谱分析,1988年,8卷,3期,51页
  • 3宋子台,环境化学,1988年,7卷,4期,45页

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