摘要
Background There may be dysregulation of circulating microRNAs in acute myocardial infarction （AMI）, which is an aging-related process. However, the difference between young and elderly people in expression level of circulating miR-21 in AMI patients has not been investigated. Methods The study included 72 consecutive patients with AMI. The group I consisted of 43 patients aged equal to or above 65 years and the group II consisted of 29 patients aged equal to or below 45 years. Real-time RT-PCR was applied to detect serum miR-21 expression levels at the time of mechanical reperfusion and 12 h, D1, D3 and D7 after PCI, respectively. Results The expression level of miR-21 in AMI patients increased markedly 12 h after PCI and reached the peak at D1 after PCI in both groups. There was no difference of miR-21 expression between Group I and U at the time of mechanical reperfusion （5.12 _＋ 0.73 vs. 4.98 ＋ 0.87） and D7 after PCI （1.28 __ 0.75 vs. 1.94 ＋ 0.89）, However, group I patients exhibited higher miR-21 expression level than group I1 at 12 h （7.96 ＋ 0.78 vs. 4.23 0.77, P 〈 0.05）, D1（9.32 __ 0.89 vs. 6.12 __ 0.92, P 〈 0.05） and D3 （4.78 __ 0.91 vs. 2.97 _＋ 0.77, P 〈 0.05） after PCI, respectively. Conclusion Our data reveal an increase of miR-21 in patients with AMI may be a mechanism of myocardial ischemia reperfusion injury. The expression of miR-21 was related to the development and progression of AMI, and there is an age-related change in the expression of miR-21 in acute myocardial infarction patients.
Background There may be dysregulation of circulating microRNAs in acute myocardial infarction （AMI）, which is an aging-related process. However, the difference between young and elderly people in expression level of circulating miR-21 in AMI patients has not been investigated. Methods The study included 72 consecutive patients with AMI. The group I consisted of 43 patients aged equal to or above 65 years and the group II consisted of 29 patients aged equal to or below 45 years. Real-time RT-PCR was applied to detect serum miR-21 expression levels at the time of mechanical reperfusion and 12 h, D1, D3 and D7 after PCI, respectively. Results The expression level of miR-21 in AMI patients increased markedly 12 h after PCI and reached the peak at D1 after PCI in both groups. There was no difference of miR-21 expression between Group I and U at the time of mechanical reperfusion （5.12 _＋ 0.73 vs. 4.98 ＋ 0.87） and D7 after PCI （1.28 __ 0.75 vs. 1.94 ＋ 0.89）, However, group I patients exhibited higher miR-21 expression level than group I1 at 12 h （7.96 ＋ 0.78 vs. 4.23 0.77, P 〈 0.05）, D1（9.32 __ 0.89 vs. 6.12 __ 0.92, P 〈 0.05） and D3 （4.78 __ 0.91 vs. 2.97 _＋ 0.77, P 〈 0.05） after PCI, respectively. Conclusion Our data reveal an increase of miR-21 in patients with AMI may be a mechanism of myocardial ischemia reperfusion injury. The expression of miR-21 was related to the development and progression of AMI, and there is an age-related change in the expression of miR-21 in acute myocardial infarction patients.
基金
supported by Science and Technology Planning Project of Guangdong Province(No.2011B031800004)
