摘要
目的:研究缺血性心力衰竭气虚血瘀证和阳虚水停证的血浆代谢组学特征,探索代谢组学在缺血性心力衰竭中医证候中的应用。方法:收集缺血性心力衰竭气虚血瘀证和阳虚水停证患者各18例,利用液相色谱质谱(LC/MS)联用技术对样本进行检测,用主成分分析(PCA)及偏最小二乘分析判别法(PLS-DA)处理图谱数据,研究两组血浆代谢物图谱的差异。结果:两组患者血浆LC-MS图谱的PCA及PLS-DA分析显示,气虚血瘀证和阳虚水停证能够被明显区分,两组血浆代谢物含量存在明显差异。缺血性心力衰竭阳虚水停证较气虚血瘀证,3种溶血磷脂酰胆碱类物质含量降低,3种肉碱类物质含量升高,2中脂肪酸类物质含量升高,肌酐含量升高。结论:缺血性心力衰竭阳虚水停证和气虚血瘀证具有不同的代谢模式,两组能量代谢及磷脂代谢存在差异。以LC-MS为技术特点的代谢组学研究方法能够将两者区分,为中医证候的客观诊断提供了新的检测方法。
Objective： To investigate metabolomics characteristics of serum samples in ischemia heart failure with syndrome of blood sta- sis caused by qi deficiency（ QDBS） and syndrome of fluid accumulation caused by Yang Deficiency（ YDFA） and explore the applica- tion of metabolomics in study of TCM syndrome of ischemia heart failure. Methods： The serum samples of ischemia heart failure with QDBS（ n = 18） and YDFA（ n = 18） were detected by Liquid chromatography-mass spectrometry（ LC-MS） and analyzed based on Prin- cipal Component Analysis（ PCA） and Partial Least Squares Discriminant Analysis（ PLS-DA）. Results： Ischemia heart failure with QDBS and YDFA can be distinguished clearly by PLS-DA. There was significant difference in metabolic characteristic between the two groups. Compared with QDBS,3 metabolites belonged to Lysophosphatidylcholines decreased in YDFA; 3 metabolites belonged to carnitines and 2 metabolites belonged to fatty and creatinine increased in YDFA. Conclusion： Energy metabolism and phospholipid metabolism were different between ischemia heart failure with QDBS and YDFA. Metabolomics analysis based on LC / MS can distinguish the two groups and provide new detective method for TCM syndromes differentiation objective.
出处
《世界中医药》
CAS
2013年第12期1393-1396,共4页
World Chinese Medicine
基金
国家自然科学基金资助项目(编号:81072930)
关键词
缺血性心力衰竭
气虚血瘀证
阳虚水停证
代谢组学
Ischemia heart failure
Syndrome of blood stasis caused by qi deficiency
Syndrome of fluid accumulation caused by Yang Deficiency
Metabolomics
