摘要
目的:观察豚鼠对脉络宁注射液、清开灵注射液和香丹注射液诱导的主动全身过敏反应(ASA)的表现,联合过敏反应相关指标的检测,综合评价中药注射剂的潜在致敏性。方法:按照《中药、天然药物免疫毒性研究的技术指导原则》的试验方法对以上3种中药注射液进行主动全身过敏试验研究,并且采用酶联免疫吸附试验(ELISA)法检测豚鼠血清总IgE和组胺水平的变化。结果:脉络宁注射液组豚鼠呈现强阳性过敏反应症状,清开灵注射液和香丹注射液组仅有少数豚鼠出现阳性过敏反应症状。与阴性对照组或药物致敏前比较,各注射液组豚鼠给药后血清总IgE或组胺水平显著升高(P<0.05或P<0.01)。结论:对于脉络宁注射液、清开灵注射液和香丹注射液3种中药注射液而言,增加过敏指标的检测更能全面评价其潜在致的敏性。
OBJECTIVE To comprehensively evaluate potential hypersusceptibility of Mailuoning, Qingkailing and Xiangdan injections by observing active systemic anaphylaxis （ASA） of guinea pigs combined with detection of related indicators. METHODS According to the guideline for immunity toxicity study on Chinese traditional medicine and natural medicine, the ASA tests were carried out. Serum IgE and histamine were determined by using ELISA. RESULTS Guinea pigs in MLN group showed strong anaphylactic symptoms, while only a few in QKL and XD groups demonstrated positive symptoms. Moreover, compared with negative control group or before sensitization, higher levels of serum IgE and histamine were found both in all injections groups after challenged （P〈0. 05,P〈0. 01 ）. CONCLUSION Increasing the observations on the allergy indexes are needed for more comprehensive assessment of the potential ASA induced by MLN, QKL and XD injections.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2014年第3期186-189,共4页
Chinese Journal of Hospital Pharmacy
基金
湖南省科技厅重点项目(编号:2011TT1001)
湖南省自然科学基金项目(编号:11JJ4079)
