摘要
以赤子爱胜蚓(Eisenia foetida)为受试生物,通过滤纸接触法和自然土壤法研究邻苯二甲酸二甲酯(Dimethyl Phthalate,DMP)、邻苯二甲酸二乙酯(Diethyl Phthalate,DEP)和邻苯二甲酸二正丁酯(Di-n-butyl Phthalate,DBP)的急性毒性效应.结果表明,DEP和DMP对赤子爱胜蚓毒性显著,滤纸接触法染毒48 h时,DMP和DEP的半数致死剂量(LD50)分别为129.603μg·cm-2和145.336μg·cm-2.自然土壤法染毒14 d时,DMP和DEP的LC50分别为1 560.120 mg·kg-1和1 516.186 mg·kg-1.DBP的LD50尚无确切数据.自然土壤法研究DMP、DEP与DBP对蚯蚓组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和乙酰胆碱酯酶(AChE)活性的影响.结果表明,SOD、CAT和AChE活性对DMP、DEP和DBP的毒性响应各不相同,其中,DMP、DEP和DBP的浓度对SOD、CAT和AChE活性呈显著性影响.SOD活性被DMP和DEP诱导增加.CAT的活性在DEP和DBP低浓度下诱导高浓度作用下抑制,而AChE活性在DEP和DBP低浓度下抑制高浓度下诱导.处理时间对SOD、AChE活性无显著性影响.
The acute toxic effects of DMP, DEP and DBP to earthworms （Eisenia foetida） were examined in this study. The two pollutants are tested by filter paper contact method and natural soil method, results indicated that they were both poisonous to earthworms. Earthworms were exposed to DMP and DEP for 48 h by filter paper contact method, LCs0 （DMP） is 129. 603 μg·cm-2and LCs0（DEP） is 145.336 μg·cm-2 Earthworms were exposed to DMP and DEP for 14 d by natural soil method, LCs0（DMP） is 1 560. 120 mg.kg-z and LCs0 （DEP） is 1 516. 186 mg-kg-1. While non-calculatable for the LCs0 of DBP due to its low water solubility, penetrating into the skin and ceils of earthworm is not easy. The natural soil test method was used to study the effects of DMP, DEP and DBP on enzyme activities of earthworms, activities of superoxide dismutase （SOD）, catalase （CAT） and acetylcholinesterasc （ACHE） were measured. SOD activity was induced by DMP and DEP, CAT is the most sensitive and the activity was induced by DEP and DBP at the low concentration and inhibited at the high concentration. AChE activity was inhibited by DEP and DBP at the low concentration and induced at the high concentration, finally exhibited a tendency to recover to the control level at 28 d.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2014年第2期770-779,共10页
Environmental Science
基金
国家自然科学基金项目(20977087)
浙江省自然科学基金项目(LY13B070008)
浙江省高校中青年学科带头人资助计划项目(2013)
浙江省公益技术研究社会发展项目(2010C33G2020078)
关键词
邻苯二甲酸酯类
赤子爱胜蚓
污染胁迫
急性毒性
抗氧化酶
phthalate acid esters
Eisenia foetida
pollution threat
acute toxicity
antioxidant enzymes
