摘要
目的观察逍遥散对雷公藤水煎剂致大鼠急性肝损伤的防治作用。方法 40只SD大鼠随机分为5组:正常组、模型组、逍遥散预防组、逍遥散对抗组、逍遥散治疗组。分别给予相应的药物干预后,采用称重法测定大鼠肝脏指数,干化学法检测血清ALT、AST活力,HE染色观察肝组织病理改变并进行半定量分析。结果与正常组比较,模型组大鼠肝脏指数及血清ALT、AST的活力明显升高,肝脏病理改变明显;与模型组比较,逍遥散预防组大鼠肝脏指数及血清ALT、AST的活力明显降低,肝脏病理损伤明显减轻,逍遥散对抗组大鼠的肝脏指数及血清AST的活力均明显降低,肝脏病理损伤明显减轻,但其ALT的活力变化不明显,逍遥散治疗组肝脏指数明显降低,肝脏病理损伤明显减轻,但其血清ALT、AST的活力变化不明显。结论提前给予逍遥散对雷公藤所致肝损伤的保护作用最为明显,表明预防作用效果最好。
Objective To observe the preventive effect of Xiaoyao Powder on acute liver injury caused by Triptergium Wilfordii Decoction in the rats. Methods Forty SD rats were randomized into 5 groups ： a normal group, a model group, Xiaoyao Powder prevention group, Xiaoyao Powder control group, Xi- aoyao Powder treatment group. After intervention with corresponding medicine, the weight method was used to determine liver index of rat. Chemical assay was used to detect the activity of ALT and AST in serum. HE staining was used to observe the pathological changes of liver tissue and semi - quantitative analysis. Results Compared with the normal group, the results of liver index and ALT and AST activities were increased ap- parently in the model group and the pathological changes in the liver were obvious. Compared with the model group, in Xiaoyao Powder prevention group, the resuhs of liver index and ALT and AST activities were re- duced apparently and the pathological injury in the liver was alleviated apparently;in Xiaoyao Powder control group, the results of liver index and AST activity were reduced apparently, the pathological injury in the liver was alleviated apparently and ALT activity did not change apparently;in Xiaoyao Powder treatment group, liv- er index was reduced apparently, liver pathologic injury was alleviated obviously, but the changes in ALT and AST activities were not obvious. Conclusion The early application of Xiaoyao Powder achieves the most ap- parent protective effect on liver injury caused by Triptergium Wilfordii,indicating the best preventive effect.
出处
《世界中西医结合杂志》
2014年第1期27-29,49,共4页
World Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金面上项目(30873433)
山西省青年科技研究基金项目(2012021031-6)
山西省高校科技开发项目(20101106)
关键词
逍遥散
雷公藤
肝损伤
防治作用
Xiaoyao Powder
Triptergimn Wilfordii
Liver Injury
Preventive Effect
