摘要
采用线粒体控制区和微卫星DNA分子标记,对梧州繁殖中心圈养黑叶猴的遗传多样性、圈养个体来源地以及个体间的亲缘关系进行分析.在355 bp线粒体控制区序列中发现了35个核苷酸变异位点,包括3个转换、29个颠换和3个插入/缺失,这些变异位点共定义了13种单倍型.圈养黑叶猴核苷酸多样性(π)为0.027,单倍型多样性(h)为0.627.采用11对微卫星引物对圈养黑叶猴DNA进行扩增,共检测到47个等位基因,每个座位的平均等位基因数为4.18.平均期望杂合度(He)为0.559,观测杂合度(Ho)为0.551.与其他濒危灵长类动物相比,圈养黑叶猴遗传多样性处于中等水平.将贵州、广西、越南野生黑叶猴和圈养黑叶猴单倍型进行比较,发现梧州繁殖中心圈养黑叶猴个体来自广西境内和广西与越南交界地区.采用微卫星数据分析了圈养黑叶猴亲缘关系和遗传距离,选取3只雄猴和7只雌猴组建了3个家庭单元用于野外放归.
Mitochondrial DNA （mtDNA） control region and microsatellite DNA sequences were used to analyze genetic diversity, origins, and relationships of captive langur （Trachypithecus francoisi） individuals. Sequencing of the 355-bp mtDNA control region for 52 individuals uncovered 35 variable nucleotide sites, including 3 transitions （ts）, 29 transversions （tv）, and 3 insertion/deletions, with 13 defined haplotypes. A haplotype diversity of 0.627 and a nucleotide diversity of 0.027 were calculated. Eleven microsatellite loci showing good amplification were also assayed in the 52 individuals. A total of 47 alleles were detected, with an average of 4.18 per locus. Mean polymorphic information content （PIC） was 0.566. Expected heterozygosity （He） and observed heterozygosity （Ho） were 0.559 and 0.551. Compared with other endangered primates, we found that the genetic diversity of the captive T. francoisi population was not low. Based on phylogenetic analysis of haplotypes of wild and captive T. francoisi individuals, we inferred that the captive T. francoisi individuals came from Guangxi, near the Vietnamese border. Using genetic distance relationships, we selected three male and seven female captive langurs to establish three family units for reintroduction.
出处
《科学通报》
EI
CAS
CSCD
北大核心
2014年第6期529-536,共8页
Chinese Science Bulletin
基金
广西壮族自治区林业厅和国家林业局珍稀濒危物种野外救护与繁育项目资助
关键词
圈养黑叶猴
微卫星DNA
遗传多样性
野外放归
captive Trachypithecusfrancoisi, microsatellite DNA, genetic diversity, reintroduction
