摘要
目的研究骨桥蛋白(OPN)在胃癌组织中的表达及临床意义。方法应用实时荧光定量PCR法检测15例新鲜原发性胃癌组织及其癌旁正常胃组织中OPN mRNA表达;免疫组化法检测69例胃癌组织、20例胃黏膜不典型增生组织及20例正常胃黏膜组织中OPN的表达水平,并探讨其表达与肿瘤临床病理参数的关系。结果胃癌组织中OPN mRNA表达高于正常胃组织(2.0832±1.1557vs.0.4640±0.1023)(P<0.01)。胃癌组织中OPN阳性表达率高于胃黏膜不典型增生组织和正常胃黏膜组织(69.6%vs.40.0%和30.0%)(P<0.05)。OPN表达与肿瘤的浸润深度、临床TNM分期和淋巴结转移有明显的相关性。结论 OPN高表达可能促进了胃癌的发病过程,在胃癌的发生发展过程中具有重要作用。
Objective To investigate the expression and clinical significance of osteopontin （OPN） in gastric cancer. Methods The mRNA expression of OPN was detected by quantitative RT- PCR in 15 cases of fresh primary gastric cancer and its tumor-adjacent normal tissues. The protein expression of OPN was determined by immunohistochemistry in 69 cases of gastric cancer, 20 cases of gastric mucosal dysplasia and 20 cases of normal gastric mucosa specimens. The relationship between the OPN expression and clinicopathological parameters was analyzed. Results The rnRNA expression of OPN in gastric cancer tissues was higher than that in tumor-adjacent normal tissues（2. 0832±1. 1557 vs. 0. 4640± 0. 1023）（P〈0. 01）. The positive expression rate of OPN in gastric cancer specimens was higher than that in gastric mucosal dysplasia and norma gastric mucosa specimens （69.6% vs. 40. 0% and 30. 0%） （P〈0. 05）. Moreover, the protein expression of OPN was closely correlated with invasion depth,TNM stage and lymph node metastasis. Conclusion High expression of OPN may promote the pathogenesis of gastric cancer, which plays an important rote in the development and progression of gastric cancer.
出处
《江苏医药》
CAS
北大核心
2014年第2期169-171,共3页
Jiangsu Medical Journal
基金
盐城市医学科技发展计划项目(YK2011129)
关键词
骨桥蛋白
胃癌
Osteopontim Gastric cancer
