摘要
目的 提高完善调经活血胶囊质量标准.方法 采用薄层色谱法对丹参、熟地黄进行鉴别;采用高效液相色谱法测定赤芍中芍药苷含量,色谱柱为Agilent Zorbax-SB C18（250mm×4.6mm,5μm）,流动相为甲醇-水（25：75）,柱温：35℃,流速：0.8mL·min-1,检测波长：230nm.结果 薄层色谱斑点清晰,分离效果良好;芍药苷对照品在0.01201～2.156μg范围内线性关系良好（r=0.9996）,平均回收率为96.61%,RSD为1.21% （n=9）.结论 本法简便、可靠、准确,可用于该制剂的质量控制.
OBJECTIVE To improve quality standard of Tiaojing Huoxue Capsules. METHODS Salviae Milt- iorrhizae Radix et Rhizoma and Rehmanniae Radix Praeparata were identified by TLC;The content of peoniflorin in Paeoniae Radix Rubra was determined by HPLC. An Agilent Zorbax-SB Cls （4. 6 mm × 250 mm,5μm） column was eluted with mobile phase consisted of methanol-water（25：75 ） at the column temperature of 35℃. The flow rate was 0. 8mL · min-1 and detective wavelength was 230nm. RESULTS The spots in TLC were quite clear. The linear range of peoniflorin was within 0. 01201 ~ 2. 156μg（ r = 0. 9996）, the average recovery of peoniflorin was 96. 61% with RSD of 1.21% （ n = 9）. CONCLUSION The established method is simple, reliable, accurate and can be used as the quality control of Tiaojing Huoxue Capsules.
出处
《海峡药学》
2014年第1期53-55,共3页
Strait Pharmaceutical Journal
基金
国家药典委员会编国家药品质量标准提高行动计划项目(编号:618)
