摘要
蜡质芽孢杆菌AR156是一种防治多种土传病害的生物制剂,对水稻纹枯病具有较好的防治效果。本研究通过温室试验方面验证菌株AR156的防病效果和促生作用,从细胞水平和基因水平初步揭示了菌株AR156防治水稻纹枯病的机理。结果表明,菌株AR156对水稻纹枯病的温室防效达73.06%,同时促进水稻生物量增加14.45%。菌株AR156处理提高了水稻植株SOD、PAL、POD和CAT等防御酶活性,增强了OsPR1b、OsPR10、OsNPR1和ZB8等防卫相关基因的表达。接种立枯丝核菌Rhizoctonia solani HNW-21之前用菌株AR156预处理的水稻植株,SOD和PAL分别提前4、2 d出现活性峰;防卫相关基因均提前表达,且表达时间延长,从而提高水稻对纹枯病的抗性。
Bacillus cereus AR156, a biocontrol agent against a variety of soil-borne pathogens, could effectively control rice sheath blight in field. Here, the biocontrol efficacy and growth-promoting role of strain AR156 on rice was tested under greenhouse conditions, and the primary mechanism of strain AR156 in controlling rice sheath blight was also studied on both cellular and gene levels. The results revealed that AR156 could control Rhizoctonia solani with an efficiancy of 73.06%, and there was a 14.45% biomass increase in AR156-treated rice plants compared with those non-treated ones. Strain AR156 increased the activities of defensive enzymes SOD, PAL, POD and CAT, and the expression of defense related genes OsPR1b、OsPR10、OsNPR1 and ZB8. In rice plants pretreated with strain AR156 before inoculated with R. solani HNW-21, SOD or PAL activity peak reached in advance by four and two days, respectively, and the accumulation of defense related genes transcripts was rapid and extended. As a result, the resistance of those plants to rice sheath blight was strengthened.
出处
《中国生物防治学报》
CSCD
北大核心
2014年第1期107-112,共6页
Chinese Journal of Biological Control
基金
国家自然基金项目(31171809
30971956)
江苏省科技成果转化专项资金(BA2012059)
