摘要
以干酪乳杆菌为研究对象,考察了酸胁迫对细胞膜生理特性的影响。研究结果表明,随着pH的降低,细胞膜侧向扩散系数降低,微黏度增加。同时分析细胞内膜透性和胞内金属离子浓度表明酸胁迫引起细胞膜透性增加。分析膜脂肪酸成分结果表明,酸胁迫引起不饱和脂肪酸含量增加,饱和脂肪酸含量降低,同时细胞膜脂肪酸不饱和度和膜脂碳链长度也相应增加。研究结果将为进一步解析干酪乳杆菌酸胁迫抗性机制奠定理论基础。
In this study,effects of acid stress on membrane characteristics were investigated by using Lactobacillus casei as initial strain. The results showed that lateral diffusion coefficient decreased and the micro-viscosity increased with the decrease of pH.In addition,analysis of the inner membrane permeability and concentrations of intracellular metal ions showed that acid stress led to increased permeability of cell membrane.The distribution of membrane fatty acids was investigated, and the results showed acid stress induced increased unsaturated fatty acids and decreased saturated fatty acids.Moreover, acid stress resulted in increased unsaturation degree and chain length of membrane fatty acids. Results presented in this study provided theoretical basis for further elucidation of the acid tolerance mechanisms employed by lactic acid bacteria.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第5期122-125,共4页
Science and Technology of Food Industry
基金
国家自然科学基金(31301546)
关键词
干酪乳杆菌
酸胁迫
细胞膜流动性
膜脂肪酸
Lactobacillus casei
acid stress
membrane fluidity
membrane fatty acid
