摘要
该实验采用高效液相色谱法,Agilent Extend-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相0.5‰三氟乙酸-乙腈,梯度洗脱,建立了不同产地及批号的荆芥防风药对的特征图谱,标定了12个共有特征峰,并得到了荆芥防风药对的对照特征图谱。该法简便,准确,重复性好,可作为该药对的质量控制依据之一。
In this experiment, the HPLC specific chromatogram was adopted, with Agilent Extend-C18 （4. 6 mm × 250 mm, 5 um） as the chromatographic column, and 0. 5‰ trifluoroacetic acid and acetonitrile as the mobile phase for gradient elution, so as to establish specific chromatograms for drug pair of Schizonepetae Herba and Saposhnikoviae Radix from different producing area, identify 12 common characteristic peaks, and obtain the comparison specific chromatography of drug pair of Schizonepetae Herba and Saposhnikoviae Radix. The method is simple, accurate and highly reproducible, and thus can be used as the basis for the quality control of the drug pair.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第4期679-683,共5页
China Journal of Chinese Materia Medica
基金
国家中医药管理局中医药行业科研专项(2010070121-7)
江苏省中医药局科技项目(LZ11198)
江苏高校优势学科建设工程项目(YSXK-2010)
南京中医药大学中药学一级学科开放课题(2011ZYX1-009)
关键词
荆芥
防风
药对
特征图谱
Schizonepeta Herba
Saposhnikovia Radix
drug pair
specific chromatogram
