摘要
目的:建立冰片-葛根素脂质体中葛根素含量及包封率测定方法。方法:采用薄膜分散法制备冰片-葛根素脂质体,葡聚糖凝胶柱层析法分离脂质体和游离药物,采用高效液相色谱法测定脂质体中葛根素的含量和包封率。结果:葛根索在8~160μg·ml^(-1)范围内线性关系良好(r=0.999 8);柱层析法可有效分离药物,分离游离药物的柱回收率为99.32%(RSD=1.16%,n=9),加样回收率为98.56%(RSD=1.23%,n=9)。3批样品的平均包封率为62.35%,65.41%,63.18%(n=3)。结论:该方法准确、灵敏,可用于冰片-葛根素脂质体中葛根素含量及包封率的测定。
Objective： To establish a method for the determination of entrapment efficiency and content of borneol-puerarin lipo- somes . Methods： Borneol-puerarin liposomes were prepared by a film dispersion method. The puerarin content and entrapment effi- ciency was determined by HPLC using a Sephadex G-50 chromatographic column. Results： The puerarin content was linear within the range of 8 - 160 μg·ml^-1 （ r = 0. 999 8 ）. The column recovery was 99.32% （ RSD = 1.16%, n = 9 ）, and the sample recovery was 98.56% （ RSD =1.23 %, n = 9）. The entrapment efficiency of three hatches of the liposomes was 62.35 %, 65.41% and 63.18% （ n = 3 ）, respectively. Conclusion： The method is accurate and sensitive, and can be used in the content and entrapment efficiency de- termination of borneol-puerarin liposomes.
出处
《中国药师》
CAS
2014年第1期16-19,共4页
China Pharmacist
基金
湖北省自然科学基金课题(编号:2012FFB04329)
关键词
冰片
葛根素
脂质体
包封率
Borneol
Puerarin
Liposomes
Entrapment efficiency
