摘要
目的:建立蛭芪康胶囊的质量控制方法。方法:采用薄层色谱法对制剂中的天麻蜜环菌粉、大黄、黄芪进行定性鉴别。采用3,5-二硝基水杨酸光度法(DNS)测定制剂多糖含量,采用Folin-酚法(Lowry法)测定制剂肽含量。结果:定性鉴别检出制剂中的天麻蜜环茵粉、大黄、黄芪;DNS法测定结果,多糖在6.412—32.060μg·ml-1的范围内呈良好的线性关系(r=0.9995),平均回收率为95.86%,RSD为0.86%(n=6);Folin-酚法测定结果,肽在0.0597~0.2984mg·ml-1范围内呈良好的线性关系r=0.9990),平均回收率为100.3%,RSD为1.88%(n=6)。结论:本方法简便、准确,可作为该制剂的质控方法。
Objective : To establish the quality control of Zhiqikang capsules. Methods : TLC was used to identify Gastrodia tuder halimasch, rhubarb and Astragalus mongholicus in the preparations. A speetrophotometry'method with 3, 5-dinitrosalicylic acid (DNS) was used to measure the polysaceharide content in Zhiqikang capsules. A spectrophotometry method with Forint phenol method (Lowry) was used to measure the peptide content in the capsules. Results: The linear range of polysaecharide was obtained between 6. 412 and 32. 060μg·ml-1(r = 0. 999 5), the average recovery was 95.86% and RSD was 0.86%. The linear range of peptide was ob- tained between 0.059 7 and 0.298 4 mg·ml-1 (r =0.999 0), the average recovery was 100.3% and RSD was 1.88% (n =6). Conclusion: The assay method is simple and accurate in the quality control of the preparations.
出处
《中国药师》
CAS
2014年第2期221-224,共4页
China Pharmacist