摘要
以宁玉草莓匍匐茎为外植体,进行消毒灭菌、茎尖分化、继代增殖和生根培养基筛选研究,结果表明:最佳消毒方法为75%乙醇40 s+0.1%升汞6~8 min;草莓茎尖分化培养基:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L;继代增殖培养基:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,其增殖系数为9;生根培养基:1/2MS+NAA 0.05 mg/L,生根率可达96.6%。
The stolons of strawberry variety “Ningyu” were used as explants .After disinfection , we studied and selected the appropriate culture media for stem -tip differentiation, subcultural propagation and rooting .The results showed that the best method of disinfection was using 75%ethanol for 40 s plus 0.1%HgCl2 for 6~8 min;the best differentiation medium was MS +0.5 mg/L 6-BA+0.1 mg/L NAA;the best medium for subcultural propagation was MS +0.5 mg/L 6-BA+0.1 mg/L NAA, and its multiplication coefficient was 9;the best rooting medium was 1/2MS+0.05 mg/L NAA, and the rooting rate reached 96.6%.
出处
《江西农业学报》
CAS
2014年第3期16-17,21,共3页
Acta Agriculturae Jiangxi
基金
农业科技成果转化资金项目(2012GB2C100159)
关键词
宁玉草莓
组织培养
快繁技术
“Ningyu” strawberry
Tissue culture
Rapid propagation