摘要
目的研究参黄液对大鼠肢体动脉缺血的治疗作用及机制。方法采用股动脉注射月桂酸的方法建立大鼠后肢缺血模型,将90只造模成功的Wistar大鼠随机分为空白对照组(A组)、75%乙醇治疗组(B组)和参黄液治疗组(C组),每组30只,A组不做任何处理,B、C组分别用75%乙醇、参黄液浸泡造模侧后肢,早晚各1次,每次15 min。于造模后第21天观察大鼠后肢缺血程度;取后肢肌肉标本行平均微血管密度(MVD)检测,应用免疫组化SABC法检测血管内皮生长因子(VEGF)相关抗原的表达情况。结果与A、B组比较,C组造模侧后肢缺血程度明显减轻(P<0.05,P<0.01),MVD及VEGF蛋白表达水平均明显升高(P<0.05);A、B组造模侧后肢缺血程度、MVD及VEGF蛋白表达水平差异无统计学意义(P>0.05)。结论参黄液可以调节VEGF蛋白的表达,促进毛细血管生成,有效改善大鼠肢体动脉缺血,防止肢端坏疽和继发感染。
Objective To study the therapeutic effect and mechanisms of Senhuang liquid in treatment of limb ischemias in rats. Methods The models of hind limbs ischemia were established by lauric acid of femoral arteries injec- tion in 90 rats, and the 90 rats modelled successfully were randomly divided into control group （ group A, n = 30） , group B （ n = 30） and group C （n = 30）. Group A was left untreated, latero-hind limbs of models were respectively soaked into 75% Alcohol liquid in group B, and Senhuang liquid in group C （15 rain each time, in the morning and at night/1 d）. The ischemic degrees of hind limbs were observed on 21＇hd after the operation; muscle specimens of hind limbs were re- moved, microvessel densities （MVD） were detected, and the expressions of vascular endothelial growth factor （VEGF） in protein level were also detected by immunocytochemistry strept avidin-biotin complex method. Results Compared with those in group A and B, in group C the ischemic degrees of hind limbs were significantly relieved （P 〈0. 05, P 〈 0.01 ） , and the expressions of MVD and VEGF were significantly increased （P 〈 0.05） ; the differences in latero-hind limbs of model ischemia degrees, expressions of MVD and VEGF were no statistically significant （ P 〉 0. 05 ）. Conclu- sion Senhuang liquid can regulate the VEGF expression, promote capillary angiogenesis, effectively improve limb ische- mias in rats and prevent the gangrene and secondary infection of the acra.
出处
《解放军医药杂志》
CAS
2014年第2期40-43,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
山东省自然科学基金课题(ZR2012HM063)
山东大学大学生科技创新基金课题(201310422093)
关键词
参黄液
投药
皮肤
肢体
缺血
毛细血管
血管内皮生长因子
大鼠
Wistar
Senhuang liquid
Drug administration, skin
Limb, ischemia
Capillary
Vascular endothelialgrowth factor
Rat, Wistar
