摘要
目的探讨白介素-1β(IL-1β)对人牙周膜成纤维细胞(hPDLFs)中基质金属蛋白酶-13(MMP-13)表达影响的研究。方法体外分离培养hPDLFs并随机分为对照组和实验组,对照组中加入等量无血清培养液,实验组加入不同浓度IL-1β(0 ng/ml、0.5 ng/ml、5 ng/ml、10 ng/ml、20 ng/ml)作用hPDLFs 24 h。采用RT-PCR检测MMP-13 mRNA的表达情况,并选取最适浓度作用24 h,采用Western blot检测MMP-13蛋白表达的变化情况。结果实验组与对照组比较,MMP-13在IL-1β浓度为10 ng/ml和20 ng/ml时mRNA表达最为明显(P<0.05),10 ng/ml组和20 ng/ml组MMP-13 mRNA表达相比较,无明显差异(P>0.05)。采用10 ng/ml IL-1β作为最适浓度进行刺激,并作用hPDLFs 24 h,与对照组比较,MMP-13蛋白表达显著增加,具有显著差异性(P<0.05)。结论在IL-1β调控下,hPDLFs中MMP-13的表达显著增加,并呈明显的浓度依赖性,而MMP-13表达的增加可能是引起正畸治疗过程中牙根吸收的重要机制之一。
Objective To investigate the effects of interleukin-1β on the expression of matrix metalloproteinase-13 （MMP-13） in human periodontal ligament fibroblasts （hPDLFs）. Methods The hPDLFs isolated and cultuered in vitro were randomly divided into control and exprimental groups. Serum-free medium was added in the control group, while different concentration of IL-1β（ 0 ng/ml, 0.5 ng/ml,5 ng/ml, 10 ng/ml,and 20 ng/ml）was added in the exprimental groups tO culture hPDLFs for 24 h. Then real-time PCR was used to detect the expression of MMP-13 mRNA, and the optimal concentration was selected to culture hPDLFs for 24 h. The changes of the expression of MMP-13 were detected by Western blot. Results Compared with the control group,the mRNA expression of MMP-13 in the experimental groups were most obvious when the concentration of IL-1 [3 was 10 ng/ml and 20 ng/ml（ P 〈 0.05 ） ,but there was no significant difference of the expression between the concentration of 10 ng/ml and 20 ng/ml （ P 〉 0.05 ）. The best concentration for the stimulus of IL-1β was 10 ng/ml. When hPDLFs were cultured with this concentration for 24 h, the MMP-13 protein expression increased significantly compared with that in the control group, and there were significant differences between the two groups （ P 〈 0.05 ）. Conclusion Under the regulation of IL-1β, the expression of MMP-13 in hPDLFs significantly increases and has obvious concentration dependency. The increase of MMP-13 expression may be one of the most important mechanisms of dental root absorption during the orthodontic treatment.
出处
《西南国防医药》
CAS
2014年第2期120-122,共3页
Medical Journal of National Defending Forces in Southwest China
基金
国家自然科学基金资助项目(81070870)
