摘要
为探讨复方术芩提取液体外抗猪流行性腹泻病毒(PEDV)感染Vero细胞作用效果,以Vero细胞为宿主细胞,通过检测药物抗PEDV活性,计算其IC50和治疗指数TI,并从药物对PEDV的直接灭活作用、阻滞作用、吸附抑制、穿入抑制及复制抑制5个方面探讨复方术芩抗PEDV作用机理。结果显示复方术芩提取液能明显抑制PEDV的致病变作用,其IC50为17.41mg/L,TI为23.40。各浓度术芩提取液对PEDV病毒复制过程中均有一定的抑制作用,其中直接灭活作用和复制抑制试验效果比较明显,且质量浓度C=100mg/L,与病毒对照相比差异极显著(P<0.01),质量浓度C≥25mg/L,细胞保护率均超过50%。而阻滞试验、吸附抑制试验和穿入抑制试验,药物质量浓度需要达到100mg/L才出现显著的保护作用。表明复方术芩提取液在体外抗PEDV主要是通过对病毒的直接灭活和对病毒复制抑制而达到抗病毒功效。
To investigate the antiviral effect of compound Zhuqin extract on Vero cells infected with porcine epidemic diarrhea virus（PEDV） in vitro,the Veto cells were used as host cells,anti- viral activity against PEDV,IC50 and therapeutic index TI of the compound were detected, direct inactivation, blocking effect, adsorption assay, penetration assay and reproduction inhibition as- say. were conducted to investigate the antiviral effect. The results indicated that the compound Zhuqin extract could inhibit PEDV obviously,the IC50 was 17.41 mg/L,TI was 23.40. Different concentrations of compound Zhuqin extract showed inhibition effect in the replication process of PEDV,direct inactivation and replication inhibition,the quantity of plaque of PEDV was reduced approximately at C= 100 rag/L, compared with virus group, the difference was extremely signifi- cant（P〈0.01） ,cell protection rate was more than 50% at C≥25 mg/L. While the difference was no significant until concentration reached 100 mg/L in blocking test,adsorption test and penetra- tion test. It is concluded that compound Zhuqin extract could resist PEDV infected Vero cells in vitro,and its main antiviral mechanism might be to inactivate the PEDV directly and inhibit the virus reproduction.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2014年第3期485-488,491,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31172357)
国家公益行(农业)科技专项经费资助项目(20130304-05)
关键词
复方术芩提取液
猪流行性腹泻病毒
抗病毒作用
compound Zhuqin extract
porcine epidemic diarrhea virus
antiviral activity
