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实时荧光定量PCR检测食管癌循环miRNA的研究 被引量:2

Real-time PCR in detection of plasmic miRNA in patients with esophageal cancer
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摘要 目的研究miR-424、miR-373、miR-34c、miR-129、miR-130b、miR-203、miR-9、miR-15b、miR-100、miR-145、miR-21在食管癌患者外周血的差异表达。方法采用Real-timePCR定量检测方法分析比较20例食管癌患者血液与健康对照者血液之间miR-424、miR-373、miR-34c、miR-129、miR-130b、miR-203、miR-9、miR-15b、miR-100、miR-145、miR-21表达差异情况。结果miR-424、miR-373、miR-34c、miR-203、miR-15b表达上调,miR-100表达下调。结论外周血miRNA可能成为食管癌患者早期诊断新的生物学指标。 Objective To investigate the differential expression of miR-424, miR-373, miR-34c, miR-129, miR-130b, miR-203, miR-9, miR-15b, miR-100, miR-145, miR-21 in serum of esophageal cancer. Methods The expression levels of miR-424, miR-373, miR-34c, miR-129, miR-130b, miR-203, miR-9, miR-15b, miR-100, miR-145, miR-21 were detected by real-time PCR in serum of 20 human esopha- geal cancer and 20 normal person. Results Over-expression of miR-424, miR-373, miR-34c, miR-203, miR-15b and low-expression of miR-100 were observed in serum of esophageal cancer. Conclusions miRNAs in the serum maybe a new biological indicator for diagnosis of human esophageal cancer.
出处 《中国实用医刊》 2014年第6期1-2,共2页 Chinese Journal of Practical Medicine
基金 广东省科技计划项目资助(20101096)
关键词 食管癌 实时荧光定量PCR MICRORNAS Esophageal cancer Real-time PCR MicroRNAs
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  • 1李克.影响食管癌早期诊断的相关因素[J].中国肿瘤,2005,14(12):781-784. 被引量:1
  • 2Duffy MJ. Clinical uses of tumor markers: a critical review. Crit Rev Clin Lab Sci 2001; 38:225-262.
  • 3Thomas CM, Sweep CG. Serum tumor markers: past, state of the art, and future, lnt J Biol Markers 2001; 16:73-86.
  • 4Duffy MJ. Role of tumor markers in patients with solid cancers: a critical review. Eur J lntern Med 2007; 18:175-184.
  • 5Roulston JE. Limitations of tumour markers in screening. Br J Surg 1990; 77:961-962.
  • 6Esquela-Kerscher A, Slack FJ. Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer 2006; 6:259-269.
  • 7Calin GA, Croce CM. MicroRNA signatures in human cancers. Nat Rev Cancer 2006; 6:857-866.
  • 8Chen C, Ridzon DA, Broomer A J, et al. Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 2005; 33:e179.
  • 9Tang F, Hajkova P, Barton SC, Lao K, Surani MA. MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Res 2006; 34:e9.
  • 10Hafner M, Landgraf P, Ludwig J, et al. Identification of microRNAs and other small regulatory RNAs using cDNA library sequencing. Methods 2008; 44:3-12.

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