摘要
目的 建立 RP- HPL C法分离检测大豆提取物中大豆苷元、染料木素、大豆苷、染料木苷 4成分含量的方法。方法 用 Zorbax SB- C1 8色谱柱 ,以甲醇 -乙腈 - 0 .1%磷酸 (30∶ 8∶ 6 2 )和甲醇 -乙腈 - 0 .1%磷酸 (16∶ 2 4∶ 6 0 )为流动相分别测定大豆苷元、染料木素和大豆苷、染料木苷 ,检测波长为 2 5 4nm。结果 大豆苷元、染料木素、大豆苷、染料木苷 4成分的平均回收率分别为 98.7% ,99.5 % ,98.4% ,98.6 % ;RSD分别为 1.4% ,0 .9% ,0 .9% ,1.7%(n=4)。结论 方法简便、准确。
Object A method for the determination of daidzein, genistein, daidzin and genistin in soybean extract by RP HPLC was established Methods Zorbax SB C 18 column (4 6×15 mm, 5 μm) was used Daidzein and genistein were separated with mobile phase Ⅰ∶methanol acetonitrile 0 1% phosphoric acid solution (30∶8∶62) Daidzin and genistin were separated with mobile phase Ⅱ∶methanol acetonitrie 0 1% phosphoric acid solution (16∶24∶60) The UV detection wavelength was 254 nm Results The average recoveries of daidzein, genistein, daidzin and genistin were 98 7%, 99 5%, 98 4%, 98 6% and the precision (RSD) were 1 4%, 0 9%, 0 9%, 1 7% (n=4) respectively Conclusion: The method was accurate, rapid and with good reproducibility
出处
《中草药》
CAS
CSCD
北大核心
2001年第2期118-120,共3页
Chinese Traditional and Herbal Drugs
