摘要
目的探讨建立紫苏叶中紫苏醛的提取和含量测定方法。方法以紫苏醛为指标，利用气相色谱法（GC）测定其含量。采用DM—FFAP色谱柱，初始柱温为120℃，以2％／min升温至140℃，然后以15℃／min升温至220℃，保持5min。比较索氏提取、加热回流、超声提取法的差异，并优选最佳提取方法。结果建立了紫苏醛的含量测定方法。紫苏醛浓度在19．26～192．63μg／ml范围内线性关系良好，平均回收率为101．68％，RSD=I．97％（n=6）。确定了最佳提取工艺，加入20倍量的无水乙醇溶液，在85℃水浴中回流提取1h。结论本提取及含量测定方法快速简便、结果可靠，可为紫苏叶质量控制提供科学依据。
Objective To explore the develop methods for extraction and content determination of perillaldehyde from folium Perillae. Methods Gas chromatography （GC） was applied to determine the content of perillaldehyde.DM-FFAP chromatographic column was applied,with initial temperature set at 120℃ then heated up to 140℃ at the speed of 2℃/min then heated up to 220℃ at the speed of 15℃/min,then maintaining the temperature at 220℃ for 5 minutes.The optimal extraction method was selected based on comparison of Soxhlet extraction,heat reflux extraction and ultrasonic extrac- tion. Results The study developed the method for perillaldehyde content determination.Perillaldehyde had good linear relation at the concentration of 19.26-192.63 μg/ml,with average recovery rate of 101.68%,RSD=1.97% （n=6）.The study identified the optimal extraction process：adding 20 times amount of anhydrous ethanol solution then heat reflux extrac- tion under 85℃ water bath for 1 hour. Conclusion The extraction and content determination methods developed in the study are quick,simply,convenient and reliable,providing scientific basis for quality control offolium Perillae.
出处
《中国当代医药》
2014年第7期4-7,共4页
China Modern Medicine
基金
河南省自主创新重大成果转化项目(11276409Z)
关键词
加热回流
紫苏叶
紫苏醛
气相色谱法
Heat reflux
Folium perillae
Perillaldehyde
Gas chromatography
