摘要
目的探讨实时荧光定量聚合酶链反应（FQ-PCR）检测肺炎支原体（MP）DNA在小儿肺炎诊断中的应用价值。方法采用FQ-PCR对该院742例儿科住院患儿的血清、痰液、胸腔积液、支气管灌洗液、肺泡灌洗液临床标本进行MPDNA的定量检测。结果742例患儿中MPDNA检测总阳性率为40．3％（299／742）；男、女性患儿MPDNA阳性率的差异无统计学意义（P〉0．05）；4～〈7岁、7～〈10岁、10～〈13岁、13～16岁组，MPDNA阳性率均高于0～〈4岁组（P〈O．05）；不同种类标本的阳性率不同，由高到低依次为支气管灌洗液（96．5％）、肺泡灌洗液（83．7％）、痰液（71．7％）、胸腔积液（38．9％）、血清（6．1％）。结论4岁及4岁以上儿童易感染肺炎支原体，且随着年龄增长感染率逐渐增高；支气管灌洗液标本的MPDNA阳性率较高；FQ-PCR法检测MP DNA用于肺炎支原体感染的诊断有一定价值。
Objective To investigate the clinical value of MP DNA detection in the diagnosis of pediatric pneumonia. Methods Real-time quantitative PCR was used to detect MP DNA in serum, sputum, hydrothorax, bronchial lavage fluid and bronchoalveolar lavage fluid from 742 cases of pediatric pneumonia in the hospital. Results The rate of MP DNA（＋） was 40.3% （299 cases in to- taD;the difference between male infection rate（40.1%） and female infection rate （40. 6 %）was not statistically significant （P〉0.05） ;The infection rates in groups of 4-〈7 years old,7-〈10 years old,10-〈13 years old and 13-16 years old were signifi- cantly higher than in the group of 0-〈4 years old （P〈0. 05） ;The positive rate differed from different types of samples from high to low were as follows, bronchial lavage fluid （96. 5 %）, bronchoalveolar lavage fluid （83.7 %）, sputum （71.7 %）, hydrothorax （38.9 % ）, serum （6.1 % ）. The difference of positive rate between blood samples and other types of samples was statistically signifi- cant （P〈0.05）. Conclusion Children older than 3 years old were susceptible to MP,and the infection rate gradually increased with children growing up. The positive rate of alveolar lavage fluid was significantly higher than serum. It indicated that the diagnosis of Pneumonia mycoplasma with real-time quantitative PCR has some important application value in clinic.
出处
《国际检验医学杂志》
CAS
2014年第4期416-418,共3页
International Journal of Laboratory Medicine
关键词
支原体
肺炎
聚合酶链反应
儿童
Mycoplasma,pneumoniae
polymerase chain reaction
child
