摘要
目的探讨二氧化硫（SO2）对左向右分流大鼠心肌组织内质网应激蛋白表达的影响。方法雄性SD大鼠24只，用随机数字表法分为假手术组（8只）、手术组（8只）和手术＋SO2组（8只）。对手术组和手术＋SO2组大鼠通过腹主动脉-下腔静脉穿刺建立左向右分流动物模型。手术＋SO2组于术后腹腔注射Na2SO3／NaHSO3。8周后，测定各组大鼠血氧饱和度，计算肺循环血量／体循环血量（Qp／Qs）；计算心脏质量／体质量（HW／BW）、右心室／（左心室＋室间隔）[RV／（LV＋SP）]；ELISA法测定各组大鼠右心室心肌组织I型胶原水平；采用高效液相色谱法测定各组大鼠右心室心肌组织SO2水平，分别采用实时荧光定量-多聚酶链式反应（RT-PCR）和Western blot法测定各组大鼠右心室心肌组织葡萄糖调节蛋白78（GRP-／8）、c-jun氨基末端激酶（JNK）和磷酸化JNK（p-JNK）mRNA和蛋白表达水平的变化。结果分流术后8周，手术组与手术＋SO2组Qp／Qs值均较假手术组明显升高（P均〈0．05）；手术组HW／BW、RV／（LV＋SP）及右心室心肌组织I型胶原水平较假手术组明显升高（P均〈0．05），予SO2干预后，HW／BW、RV／（LV＋SP）及右心室心肌组织I型胶原水平均明显降低（P均〈0．05）。右心室心肌组织SO2水平在手术组较假手术组明显升高，而手术＋SO2组明显高于手术组（P均〈0．05）。右心室心肌组织GRP78、JNK和P—JNK的mRNA和蛋白表达在手术组明显高于假手术组（P均〈0．05）；而应用SO2干预后，GRP78、JNK和p-JNK在mRNA和蛋白表达水平明显降低（P均〈0．05）。结论SO2可能通过降低左向右分流大鼠右心室心肌组织内质网应激蛋白表达而对心肌组织发挥保护作用。
Objective To explore the possible role of sulfur dioxide （SO2） in endoplasmic reticulum stress （ERS） protein expression in myocardial tissue of rats with left to right shunt. Methods A total of 24 male Sprague Dawley rats were randomly divided into sham group（ n = 8 ） , shunt group（ n = 8 ）and shunt plus SO2 donor group （n = 8）. The rats of shunt group and shunt plus SO2 donor group, an abdominal aorta and inferior vena cava shunting was created. Na2 SO3/NaHSO3 was administrated intra-peritoneally in rats of shunt plus SO2 donor group. Eight weeks later, the ratio of quantity of pulmonary to quantity of system（Qp/Qs） was measured;Heart weight/body weight（HW/BW） and right ventricle/left ventricle ＋ ventricular septum [ RV/（ LV ＋ SP） ] were detected;Expressions of collagen type I in right ventricle myocardial tissue was measured by enzyme linked immunosorbent assay （ELISA） ;High performance liquid chromatography was used to measure SO2 content in right ventricle myocardial tissue. Glucose regulated protein 78 （GRP78） ,c-jun N-terminal kinase（JNK） and phosphorylation-c-jun N-termina kinase（p-JNK） mRNA and protein expressions in RV myocardial tissue were detected by Western blot and real time-polymerase chain reaction（RT-PCR）. Results After 8-weeks shunting, Qp/Qs in the shunt and shunt plus SO2 v groups were higher significantly than those of the sham group（ all P 〈0.05 ）. Compared with sham group, HW/BW, RV/LV ＋ SP and expressions of collagen type I in the shunt group were significantly higher（ all P 〈 0.05 ）. Treatment of SO2, HW/BW and RV/LV ＋ SP and collagen type I in the shunt group were lower significantly（ all P 〈 0.05 ）. The level of SO2 of RV in shunt group increased significantly compared with sham group （ all P 〈 0.05 ）, but significantly lower than that of shunt plus SO2 donor group （ P 〈 0.05 ）. Compared with sham group, the expressions of GRP78, JNK and p-JNK mRNA and protein in RV increased significantly in shunt group（P 〈 0.05 ）. After treatment with SO2 ,the expressions of GRP78,JNK and p-JNK mRNA and protein decreased significantly（P 〈 0.05 ）. Conclusion SO2 might protect RV myocardial tissue of the rats with left-right shunt by regulating the expressions of ERS.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2014年第1期28-32,共5页
Chinese Journal of Applied Clinical Pediatrics
