摘要
目的:建立鹿茸草的HPLC指纹图谱,为鹿茸草的质量控制提供依据。方法:采用HPLC-UV分析鹿茸草的指纹图谱,色谱条件为Agilent HC C18色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.4%磷酸水(B)梯度洗脱,流速0.8 mL·min-1,柱温35℃,检测波长254 nm,进样量10μL。利用中药色谱指纹图谱相似度评价系统(2004A版)对不同批次鹿茸草的HPLC图进行比较分析。结果:建立了鹿茸草的HPLC指纹图谱,以木犀草素为参照峰,确立了鹿茸草指纹图谱中的13个共有峰。结论:所建立的指纹图谱稳定性和重复性均好,可用于鹿茸草药材的常规质量控制。
Objective:To establish the chromatographic fingerprint for Monochasma sheareri.by RPHPLC.Method:The HPLC fingerprint ofM.sheareri were determined on an Agilent HC C18 column (4.6 mm× 250 mm,5 μm) eluted with the mobile phase consisted of acetonitrile and 0.4% phosphoric acid in gradient mode,flow rate 0.8 mL ·min-1,column temperature 35 ℃ and the detection wavelength was set at 254 nm.The chromatograms of different batches of M.sheareri.were compared by the software of Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004A).Result:HPLC Fingerprint of M.sheareri.was established with luteolin as the reference compound.13 common peaks were selected as the fingerprint peaks of M.sheareri.Conclusion:The established HPLC fingerprint of M.sheareri.has desirable precision,reproducibility,and can be applied to routine quality control of M.sheareri.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第5期82-85,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科技计划项目(2009B0308001002)