摘要
从新疆荒漠土壤中分离筛选絮凝剂产生菌,得到一株对高岭土悬浊液具有较高絮凝活性的微生物絮凝剂产生菌W36-1,根据菌株的Biolog鉴定以及16S rRNA基因序列分析,确定为一株欧文氏菌属（Erwinia）新种。该菌絮凝产物的发酵动力学研究表明,其属于II型发酵,即菌体生长和产物合成部分偶联;在发酵48 h后絮凝活性最高,产量可达到6 g/L;絮凝特性研究表明,在20~100℃范围内有较高的温度稳定性;在酸性环境条件下有较高的絮凝活性,pH值大于7,絮凝活性迅速下降;一些金属阳离子对絮凝有促进作用,其中,Ca2＋的助凝效果最好,Mg2＋次之,Al3＋和Fe3＋的助凝效果最差。该絮凝剂对水中各种重金属离子和色素具有较好的吸附去除作用,显示出其在环境工程中良好的应用前景。
An efficient flocculant-producing strain W36-1 was isolated from desert soil in Xinjiang. Based on the Biolog microbial identification system and 16S rRNA gene sequence analysis,strain W36-1 was classified as Erwinia,and may be a novel species. Flocculant ferment of the strain indicated that the ferment belonged to type II. After 48 h culture,the highest yield of flocculation was 6 g/L. Flocculant properties showed that the flocculation had higher temperature stability at 20 ~ 100℃. The flocculating activity was higher under acidic conditions,which declined rapidly under alkaline conditions. Some metal cations could promote the flocculating activity,in which Ca2 ＋was the best,Mg2 ＋was lower than Ca2 ＋,Al3 ＋and Fe3 ＋were the lowest. Furthermore,the flocculant could absorb heavy metals and pigments efficiently,which suggested good application prospective in environmental engineering.
出处
《环境工程学报》
CAS
CSCD
北大核心
2014年第3期1208-1214,共7页
Chinese Journal of Environmental Engineering
基金
新疆自然科学基金项目(2012211A100)
国家高技术研究发展计划(863)项目(2012AA063503
2012AA021705)
