摘要
以陕西长枣皮为原料,采用超声波减压法提取其中多酚,通过单因素试验及正交试验L9(34)考察了各因素间的交互作用,优化了超声波辅助减压提取枣皮多酚的条件。在此基础上,对比了常规浸提、超声浸提和超声波辅助减压提取三者对提取得率和提取时间的影响,并通过清除自由基能力(DPPH·)衡量了各种提取方法的优劣。结果表明:超声波辅助减压提取枣皮多酚的最佳工艺参数为乙醇体积分数50%,提取温度55℃,液料比30∶1(mL∶g),超声波功率240 W,提取时间10 min,枣皮多酚得率可达5.05%,此条件下提取2次,多酚得率为6.33%,提取率即达97.37%;对比热浸提和超声波浸提,超声波辅助减压提取在明显缩短提取时间的同时又能显著提高枣皮多酚得率;清除自由基能力(DPPH·)测试表明超声波辅助减压提取所得的多酚粗提物活性最高,清除率88.4%,该方法能够更好的保护枣皮多酚的生物活性。
In order to obtain maximum yield of polyphenols from jujube peel, ultrasound-assisted vacuum extraction （UAVE） was chosen to extract the peel of Ziziphus jujube var. Changzao. The effects of various conditions were investigated by single factor experiments and orthogonal design L9 （ 34 ）. Based on the optimization condition, three extraction methods, i. e. , UAVE, ultrasound assist extraction（UAE） and conventional extraction（ CE）, were compared with both extraction time and polyphenols yield. To further evaluate UAVE, the radical scavenging activities （ DPPH ~ ） was measured, too. Results showed that ： the optimal conditions were 50 % aqueous ethanol as extraction solvent, temperature 55 ~C, solid/liquid ratio 1 ： 30 （ g ： mL）, ultrasonic power 240 W, extraction time l0 min. Under this condition, the polyphenols yield could be 5.05 % , and the extraction rate could reach 97.37 % with duplicate extraction. UAVE has advantages such as high yield and time saving by comparison with CE and UAE. The polyphenols extracted by UAVE showed that higher antioxidant activities could be obtained than those by CE and UAE. It indicated that UAVE could protect the bioactive capacity of polyphenols from jujube peel.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2014年第1期107-112,共6页
Chemistry and Industry of Forest Products
基金
国家林业局948技术引进项目(2012-4-12)
国家863计划资助(2011AA100802-9)
关键词
枣皮
多酚
超声波辅助减压提取
清除自由基
jujube peel
polyphenols
ultrasound-assisted vacuum extraction （UAVE）
radical scavenging
