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国产血竭总黄酮成分的纯化及活性成分的含量测定

Purification of total flavones by macroporous resin and its determination of the active components from Resina Draconis
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摘要 目的 采用大孔树脂对国产血竭总黄酮进行分离纯化,并测定其中活性成分的含量.方法 收集大孔树脂70%乙醇和95%乙醇洗脱液,采用高效液相色谱(HPLC)法测定其中活性成分——龙血素A、龙血素B的含量.以ODS柱为分析柱,乙腈:1%冰醋酸(34.5∶65.5)为流动相,检测波长为280 nm.结果 龙血素A、龙血素B分别在11.00 ~ 275.00 g/ml、20.00~500.00 g/ml范围内线性关系良好,线性方程分别为Y=35 844C +44 725(r =0.999 9),Y=28 533C-41 085(r =0.999 9);方法的准确度、精密度、稳定性均符合要求.制得的国产血竭精制总黄酮中龙血素A、龙血素B的含量分别为26.93、25.53 mg/g.结论 本法可用于国产血竭中相关活性成分的分析及制备,为进一步研究国产血竭活血化瘀作用提供依据. Objective To purify the total flavones from Resina Draconis using D10I resin, and set up a method of simultaneous- ly determining the contents of loureirin A and B in the extract. Methods Fractions in 70% and 95% ethanol were collected. Mixture of acetonitrile and 1% acetic acid (34.5 : 65.5) was used as the mobile phase and ODS column was used as stationary phase to determine loureirin A and B. The detecting wave length was 280 nm. Results In the established HPLC method, the linear range of loureirin A was 11.00 - 275. O0 g/ml, and that of loureirin B was 20.00 - 500.00 g/ml. Linear equation of loureirin A was Y = 35 844C + 44 725 ( r = 0. 999 9 ) and that of loureirin B was Y = 28 533 C - 41 085, r = 0. 999 9. The accuracy, precision and stability of this method were satisfactory. Conclusion The proposed method was suitable for preparation of total flavones and determination of its active compo- nents front Resina Draconis.
出处 《药学实践杂志》 CAS 2014年第1期42-44,共3页 Journal of Pharmaceutical Practice
基金 国家自然科学基金(30901981) 第二军医大学药学院"时珍学者培养计划"资助
关键词 国产血竭总黄酮 大孔树脂 total flavones in Resina Draconis macroporous resin loureirin A loureirin B HPLC
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